Development of deoxynivalenol contents in relation to the PCR detection of potentially trichothecene producingFusarium spp. during storage of wheat

Development of deoxynivalenol (DON) in wheat with a low contamination withFusarium spp. was investigated under suboptimal storage conditions (17% and 20% grain moisture, 20°C). The influence of storage on the relative DNA content of potential DON producers was also determined. The DON contents were...

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Bibliographic Details
Published in:Mycotoxin research 2000-03, Vol.16 Suppl 1 (S1), p.46-49
Main Authors: Birzele, B, Prange, A, Schönling, J, Krämer, J
Format: Article
Language:English
Subjects:
Contamination
Deoxynivalenol
Deoxyribonucleic acid
DNA
Luminous intensity
Moisture effects
Polymerase chain reaction
Storage conditions
Wheat
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Summary:Development of deoxynivalenol (DON) in wheat with a low contamination withFusarium spp. was investigated under suboptimal storage conditions (17% and 20% grain moisture, 20°C). The influence of storage on the relative DNA content of potential DON producers was also determined. The DON contents were quantified using an ELISA. The Tox5 PCR was used for the detection of potential trichothecene producers and for the estimation of their relative DNA content. ThegaoA gene was subsequently amplified by PCR to detect specificallyFusarium graminearum. The concentration ofF. graminearum DNA was semiquantitatively determined using a Light Cycler™. The DON concentrations increased during storage trials but the intensity of PCR signals decreased.
ISSN:0178-7888
1867-1632
DOI:10.1007/BF02942979