Imidazole Initiates Exsheathing of L3 Teladorsagia circumcincta

The infective larvae of Teladorsagia circumcincta have a protective sheath that is lost soon after they reach the rumen of the sheep (the definitive host). Incubation in vitro with 50 mM imidazole caused more than 75% of L₃ T. circumcincta to begin exsheathing within 2 hr. The initiation of exsheath...

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Bibliographic Details
Published in:The Journal of parasitology 2013-04, Vol.99 (2), p.332-336
Main Authors: Brown, Simon, Neale, Jennifer D, Nava-Tirado, Magnolia, Simpson, Heather V, Pedley, Kevin C, Simcock, David C
Format: Article
Language:English
Subjects:
acetic acid
Animals
Enzyme Inhibitors - pharmacology
Histamines
histidine
Imidazoles
Imidazoles - pharmacology
lactose
Larva - drug effects
Larva - physiology
Larvae
maltose
Nematode larvae
Nematodes
Parasitology
Rumen
Rumen - parasitology
Rumen bacteria
Rumen fluids
Sheep
Sheep Diseases - parasitology
Teladorsagia circumcincta
THERAPEUTICS-DIAGNOSTICS
Trichostrongyloidea - drug effects
Trichostrongyloidea - physiology
Trichostrongyloidiasis - parasitology
Trichostrongyloidiasis - veterinary
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Summary:The infective larvae of Teladorsagia circumcincta have a protective sheath that is lost soon after they reach the rumen of the sheep (the definitive host). Incubation in vitro with 50 mM imidazole caused more than 75% of L₃ T. circumcincta to begin exsheathing within 2 hr. The initiation of exsheathing was less likely at pH 6.2 than at pH 7.8. The apparent pKₐ of this process was 7.08, similar to that for the conversion of imidazolium⁺ to imidazole. Both the extent and the initial rate of exsheathing initiation increased with imidazole concentration (the apparent K½ was about 50 mM). The initial rate of exsheathing initiation was stimulated by lactose and maltose, but not by some other carbohydrates, and by propylamine and imidazole acetic acid, but not by histidine.
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-3051.1