Influence of phytic acid and its metal complexes on the activity of pectin degrading polygalacturonase

• Published in: Carbohydrate polymers 2013-06, Vol.95 (1), p.167-170
• Main Authors: Asghar, Uzma, Rehman, Haneef Ur, Qader, Shah Ali Ul, Maqsood, Zahida Tasneem
• Format: Article
• Language: English
• Subjects: aluminum; Aluminum - chemistry; Analytical, structural and metabolic biochemistry; Bacillus - enzymology; Bacillus licheniformis; Biological and medical sciences; Coordination Complexes - chemistry; copper; Copper - chemistry; enzyme inhibition; Enzymes and enzyme inhibitors; fruits; Fundamental and applied biological sciences. Psychology; Hydrolases; ions; metal ions; Metallic cations; pathogenicity; pectins; Pectins - chemistry; Phytate–metal cations complexes; Phytic acid; Phytic Acid - chemistry; plant tissues; Polygalacturonase; Polygalacturonase - chemistry; spoilage; Vanadium - chemistry; vegetables
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.02.065

► Phytic acid significantly inhibits the activity of polygalacturonase. ► The phytate-metallic complexes inhibit the polygalacturonase activity to some extent. ► 70% of polygalacturonase activity was lost after treatment of 20mM of phytic at 37°C for 30min. ► Treatment of fruits and vegetables plants to phytic acid and Al+3 might be helpful to reduce their spoilage. Polygalacturonase is one of the important requirements of different microorganism to cause pathogenicity and spoilage of fruits and vegetables that involved in degradation of pectin during plant tissue infections. In current study, 20mM phytic acid inhibited 70% activity of polygalacturonase. The effect of different concentration of metal ions such as Cu+2, Al+3 and V+4 were studied separately and it was found that the 20mM of these metal ions inhibited 37.2%, 79%, and 53% activity of polygalacturonase, respectively. Finally, the complexes of phytic acid and these metals ions were prepared and 1:1 ratio of phytic acid and metal ions complexes showed maximum inhibitory activity of enzyme as compared to complexes having 1:2 and 1:3 ratio except phytate–copper complexes which showed no inhibitory effect on the activity of polygalacturonase.