trans-10,cis-12 Conjugated Linoleic Acid Improved Growth Performance, Reduced Lipid Deposition and Influenced CPT I Kinetic Constants of Juvenile Synechogobius hasta

• Published in: Lipids 2013-05, Vol.48 (5), p.505-512
• Main Authors: Tan, Xiao-Ying, Luo, Zhi, Zeng, Qiang, Zhao, Yan-Hong, Liu, Xu
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Carnitine O-Palmitoyltransferase - metabolism; CPT I; Dietary Fats, Unsaturated - metabolism; Fish; Fish oils; Growth performance; Kinetic constants; Kinetics; Life Sciences; Linoleic Acids, Conjugated - metabolism; Lipid deposition; Lipid Metabolism; Lipidology; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Original Article; Perciformes - growth & development; Perciformes - metabolism; Synechogobius hasta; t10c12 CLA isomer
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-013-3759-5

trans -10, cis -12 (t10c12) Conjugated linoleic acid (CLA) reduced body lipid deposition in various experimental animals, but the mechanisms involved were still emerging. Carnitine palmitoyltransferase I (CPT I) catalyzes an important regulatory step in lipid metabolism. At present, no studies, to our knowledge, have evaluated the kinetic constants influenced by dietary CLA in fish. In the present study, we tested the hypothesis that changes in body lipid content in fish as a response to dietary t10c12 CLA was related to the change of CPT I kinetic constants [Michaelis constant ( K m ), maximal velocity and catalytic efficiency for carnitine and palmitoyl-CoA]. Juvenile Synechogobius hasta were fed three experimental diets with fish oil replaced with 0 (control), 1, or 2 % t10c12 CLA for 8 weeks. Weight gain, specific growth rate and protein efficiency rate increased with dietary t10c12 CLA level. Dietary t10c12 CLA addition significantly reduced lipid contents both in liver and muscle. Dietary CLA addition also improved CPT I activities in muscle but did not significantly influence hepatic CPT I activity. CPT I kinetic parameters ( K m , V max and catalytic efficiency) were significantly influenced by t10c12 CLA. CPT I catalytic efficiencies with carnitine and palmitoyl-CoA as substrates were higher in muscle and liver of fish fed increasing t10c12 CLA. For the first time, the findings demonstrated effect of dietary CLA addition on CPT I kinetics in fish and supported our starting hypothesis that dietary t10c12 CLA addition induced alterations in CPT I kinetic constants of muscle and liver. Increased CPT I catalytic efficiency might be the main reason for reduced lipid deposition in these tissues by dietary t10c12 CLA supplementation.