Association between the plasma proteome and serum ascorbic acid concentrations in humans

Vitamin C has been associated with a reduced risk of chronic diseases, but the biological pathways regulated by vitamin C are not all known. The objective was to use a proteomics approach to identify plasma proteins associated with circulating levels of ascorbic acid. Men and women (n=1022) 20–29 ye...

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Bibliographic Details
Published in:The Journal of nutritional biochemistry 2013-05, Vol.24 (5), p.842-847
Main Authors: Da Costa, Laura A, García-Bailo, Bibiana, Borchers, Christoph H, Badawi, Alaa, El-Sohemy, Ahmed
Format: Article
Language:English
Subjects:
Adult
alpha 1-Antitrypsin - blood
alpha-Tocopherol - blood
angiotensinogen
Angiotensinogen - blood
Ascorbic acid
Ascorbic Acid - blood
binding proteins
Biomarkers
blood
blood proteins
Ceruloplasmin - analysis
chronic diseases
complement
Complement C3 - analysis
Complement C9 - analysis
covariance
Cross-Sectional Studies
fasting
Female
ferroxidase
food frequency questionnaires
high performance liquid chromatography
Humans
lifestyle
Male
men
monitoring
nutrigenomics
Plasma proteins
plasminogen
proteome
Proteome - analysis
Proteomics
risk
Surveys and Questionnaires
Vitamin C
vitamin D
waist circumference
women
Young Adult
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Summary:Vitamin C has been associated with a reduced risk of chronic diseases, but the biological pathways regulated by vitamin C are not all known. The objective was to use a proteomics approach to identify plasma proteins associated with circulating levels of ascorbic acid. Men and women (n=1022) 20–29 years of age from the Toronto Nutrigenomics and Health Study completed a general health and lifestyle questionnaire and a 196-item food frequency questionnaire and provided a fasting blood sample. Circulating ascorbic acid was analyzed by high-performance liquid chromatography, and a mass-spectrometry-based multiple reaction monitoring method was used to measure 54 proteins abundant in plasma that are involved in numerous physiologic pathways. Mean protein concentrations were compared across tertiles of serum ascorbic acid using analysis of covariance adjusted for sex, ethnocultural group, season of blood draw, hormonal contraceptive use among women, waist circumference and tertiles of plasma α-tocopherol. A Bonferroni significance level of P
ISSN:0955-2863
1873-4847
DOI:10.1016/j.jnutbio.2012.05.002