A Cell Cycle-Dependent Regulatory Circuit Composed of 53BP1-RIF1 and BRCA1-CtIP Controls DNA Repair Pathway Choice

DNA double-strand break (DSB) repair pathway choice is governed by the opposing activities of 53BP1 and BRCA1. 53BP1 stimulates nonhomologous end joining (NHEJ), whereas BRCA1 promotes end resection and homologous recombination (HR). Here we show that 53BP1 is an inhibitor of BRCA1 accumulation at D...

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Bibliographic Details
Published in:Molecular cell 2013-03, Vol.49 (5), p.872-883
Main Authors: Escribano-Díaz, Cristina, Orthwein, Alexandre, Fradet-Turcotte, Amélie, Xing, Mengtan, Young, Jordan T.F., Tkáč, Ján, Cook, Michael A., Rosebrock, Adam P., Munro, Meagan, Canny, Marella D., Xu, Dongyi, Durocher, Daniel
Format: Article
Language:English
Subjects:
Binding Sites
BRCA1 Protein - genetics
BRCA1 Protein - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Cycle - genetics
DNA
DNA End-Joining Repair - genetics
DNA Repair
HEK293 Cells
HeLa Cells
homologous recombination
Humans
interphase
Intracellular Signaling Peptides and Proteins - genetics
Intracellular Signaling Peptides and Proteins - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
phosphorylation
resection
S Phase
Telomere-Binding Proteins - genetics
Telomere-Binding Proteins - metabolism
Tumor Suppressor p53-Binding Protein 1
tumor suppressor proteins
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Summary:DNA double-strand break (DSB) repair pathway choice is governed by the opposing activities of 53BP1 and BRCA1. 53BP1 stimulates nonhomologous end joining (NHEJ), whereas BRCA1 promotes end resection and homologous recombination (HR). Here we show that 53BP1 is an inhibitor of BRCA1 accumulation at DSB sites, specifically in the G1 phase of the cell cycle. ATM-dependent phosphorylation of 53BP1 physically recruits RIF1 to DSB sites, and we identify RIF1 as the critical effector of 53BP1 during DSB repair. Remarkably, RIF1 accumulation at DSB sites is strongly antagonized by BRCA1 and its interacting partner CtIP. Lastly, we show that depletion of RIF1 is able to restore end resection and RAD51 loading in BRCA1-depleted cells. This work therefore identifies a cell cycle-regulated circuit, underpinned by RIF1 and BRCA1, that governs DSB repair pathway choice to ensure that NHEJ dominates in G1 and HR is favored from S phase onward. [Display omitted] ► 53BP1 inhibits BRCA1 recruitment to DSB sites in G1 ► RIF1 is the effector of 53BP1 during DSB repair ► Class-switch recombination requires RIF1 ► RIF1 recruitment to DSB sites in S/G2 is inhibited by BRCA1-CtIP
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2013.01.001