The expression of NOTCH2, HES1 and SOX9 during mouse retinal development

• Published in: Gene Expression Patterns 2013-03, Vol.13 (3-4), p.78-83
• Main Authors: Zhu, Min-Yan, Gasperowicz, Malgorzata, Chow, Robert L.
• Format: Article
• Language: English
• Subjects: Animals; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Cell Differentiation - genetics; Development; Gene Expression Regulation, Developmental; Hes1; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Mice; Mouse; Neuroglia - cytology; Neuroglia - metabolism; Neurons - cytology; Neurons - metabolism; Notch; Receptor, Notch2 - genetics; Receptor, Notch2 - metabolism; Retina; Retina - growth & development; Retina - metabolism; Sox9; SOX9 Transcription Factor - genetics; SOX9 Transcription Factor - metabolism; Stem Cells - cytology; Stem Cells - metabolism; Transcription Factor HES-1
• ISSN: 1567-133X; 1872-7298
• DOI: 10.1016/j.gep.2012.12.001

► NOTCH2 is expressed in the early retinal neural progenitors during mouse development. ► NOTCH2 is expressed in the Müller glial cells in the postnatal and adult retina. ► The expression pattern of HES1 and SOX9 during retinal development overlaps with NOTCH2. Notch signaling is an important regulator of both developmental and post-developmental processes. In the developing retina, Notch1 is required for the maintenance of retinal progenitor cells and for inhibiting photoreceptor cell fate, while Notch3 is required for inhibiting ganglion cell fate. Here we used immunolabeling coupled with a knock-in reporter approach to obtain a detailed spatiotemporal expression pattern of Notch2 during mouse retinal development. Although previous in situ hybridization studies did not reveal appreciable levels of Notch2 in the developing retina, we detected NOTCH2 protein and reporter expression in early embryonic retinal progenitors that also expressed the Notch downstream gene, HES1. In the postnatal retina, NOTCH2, as well as the Notch downstream genes, HES1 and SOX9, were detected in VSX2/Cyclin D1/SOX2-expressing cells in the postnatal retina, and in the mature retina NOTCH2 was most abundant in Müller glia. Our findings indicate a potential role for Notch2 in the developing and mature retina.