Optimization of Seoul-Fluor-based lipid droplet bioprobes and their application in microalgae for bio-fuel study

We synthesized a series of Seoul-Fluor-based lipid droplet bioprobes with a linear range of lipophilicity and identified SF44 and SF58 as SF-based LD bioprobes in microalgae for biofuel research as well as in mammalian cells. Unlike Nile Red, SF-based bioprobes can stain algal LDs with excellent eff...

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Bibliographic Details
Published in:Molecular bioSystems 2013-05, Vol.9 (5), p.952-956
Main Authors: Lee, Youngjun, Na, Sangcheol, Lee, Sanghee, Jeon, Noo Li, Park, Seung Bum
Format: Article
Language:English
Subjects:
Animals
Biofuels
Biomass
Chlorophyta - chemistry
Chlorophyta - growth & development
Chlorophyta - metabolism
Cytoplasmic Granules - chemistry
Fluorescent Dyes - chemistry
Heterocyclic Compounds, 4 or More Rings - chemistry
Humans
Lipids - analysis
Lipids - chemistry
Microalgae - chemistry
Microalgae - growth & development
Microalgae - metabolism
Molecular Structure
Oxazines - chemistry
Reproducibility of Results
Staining and Labeling - methods
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Summary:We synthesized a series of Seoul-Fluor-based lipid droplet bioprobes with a linear range of lipophilicity and identified SF44 and SF58 as SF-based LD bioprobes in microalgae for biofuel research as well as in mammalian cells. Unlike Nile Red, SF-based bioprobes can stain algal LDs with excellent efficiency under the non-invasive and non-cytotoxic conditions.
ISSN:1742-206X
1742-2051
DOI:10.1039/c2mb25479d