Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes

• Published in: Annals of nuclear medicine 2013-04, Vol.27 (3), p.233-238
• Main Authors: Doai, Mariko, Watanabe, Naoto, Takahashi, Tomoko, Taniguchi, Mitsuru, Tonami, Hisao, Iwabuchi, Kuniyoshi, Kayano, Daiki, Fukuoka, Makoto, Kinuya, Seigo
• Format: Article
• Language: English
• Subjects: Adult; DNA Damage; Female; Histones - genetics; Humans; Imaging; Iodine Radioisotopes - adverse effects; Iodine Radioisotopes - therapeutic use; Lymphocytes - metabolism; Lymphocytes - radiation effects; Male; Medicine; Medicine & Public Health; Middle Aged; Nuclear Medicine; Original Article; Radiation Dosage; Radiology; Thyroid Neoplasms - genetics; Thyroid Neoplasms - radiotherapy
• ISSN: 0914-7187; 1864-6433
• DOI: 10.1007/s12149-012-0678-0

Purpose The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection. Methods This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining. Results The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant ( P  = 0.001