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Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes
Purpose The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection. Methods This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All...
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| Published in: | Annals of nuclear medicine 2013-04, Vol.27 (3), p.233-238 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 238 |
| container_issue | 3 |
| container_start_page | 233 |
| container_title | Annals of nuclear medicine |
| container_volume | 27 |
| creator | Doai, Mariko Watanabe, Naoto Takahashi, Tomoko Taniguchi, Mitsuru Tonami, Hisao Iwabuchi, Kuniyoshi Kayano, Daiki Fukuoka, Makoto Kinuya, Seigo |
| description | Purpose
The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection.
Methods
This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining.
Results
The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant (
P
= 0.001 |
| doi_str_mv | 10.1007/s12149-012-0678-0 |
| format | article |
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The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection.
Methods
This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining.
Results
The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant (
P
= 0.001 < 0.05). Absorbed doses estimated for the 15 patients were 0.77 ± 0.31 Gy applying standard line in vitro external radiation doses.
Conclusion
γ-H2AX foci immunodetection in lymphocytes may detect radiation-induced DNA damage associated with I-131 therapy for thyroid cancer, and may facilitate estimation of the radiation doses absorbed with this therapy.</description><identifier>ISSN: 0914-7187</identifier><identifier>EISSN: 1864-6433</identifier><identifier>DOI: 10.1007/s12149-012-0678-0</identifier><identifier>PMID: 23264066</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Adult ; DNA Damage ; Female ; Histones - genetics ; Humans ; Imaging ; Iodine Radioisotopes - adverse effects ; Iodine Radioisotopes - therapeutic use ; Lymphocytes - metabolism ; Lymphocytes - radiation effects ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Nuclear Medicine ; Original Article ; Radiation Dosage ; Radiology ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - radiotherapy</subject><ispartof>Annals of nuclear medicine, 2013-04, Vol.27 (3), p.233-238</ispartof><rights>The Japanese Society of Nuclear Medicine 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-3da9c137e85850c5588a32f2f78320e1b61be8d501bf1232531c92dc7fb7ca823</citedby><cites>FETCH-LOGICAL-c430t-3da9c137e85850c5588a32f2f78320e1b61be8d501bf1232531c92dc7fb7ca823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23264066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doai, Mariko</creatorcontrib><creatorcontrib>Watanabe, Naoto</creatorcontrib><creatorcontrib>Takahashi, Tomoko</creatorcontrib><creatorcontrib>Taniguchi, Mitsuru</creatorcontrib><creatorcontrib>Tonami, Hisao</creatorcontrib><creatorcontrib>Iwabuchi, Kuniyoshi</creatorcontrib><creatorcontrib>Kayano, Daiki</creatorcontrib><creatorcontrib>Fukuoka, Makoto</creatorcontrib><creatorcontrib>Kinuya, Seigo</creatorcontrib><title>Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes</title><title>Annals of nuclear medicine</title><addtitle>Ann Nucl Med</addtitle><addtitle>Ann Nucl Med</addtitle><description>Purpose
The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection.
Methods
This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining.
Results
The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant (
P
= 0.001 < 0.05). Absorbed doses estimated for the 15 patients were 0.77 ± 0.31 Gy applying standard line in vitro external radiation doses.
Conclusion
γ-H2AX foci immunodetection in lymphocytes may detect radiation-induced DNA damage associated with I-131 therapy for thyroid cancer, and may facilitate estimation of the radiation doses absorbed with this therapy.</description><subject>Adult</subject><subject>DNA Damage</subject><subject>Female</subject><subject>Histones - genetics</subject><subject>Humans</subject><subject>Imaging</subject><subject>Iodine Radioisotopes - adverse effects</subject><subject>Iodine Radioisotopes - therapeutic use</subject><subject>Lymphocytes - metabolism</subject><subject>Lymphocytes - radiation effects</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Nuclear Medicine</subject><subject>Original Article</subject><subject>Radiation Dosage</subject><subject>Radiology</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - radiotherapy</subject><issn>0914-7187</issn><issn>1864-6433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkcuOFCEUhonROD2jD-DGsHTDyIEqilp2xsuYTHShJu4IxaWHSRe0QBnrBXwh38Nnkk6PLo0JySHhOx8550foGdBLoHR4WYBBNxIKjFAxSEIfoA1I0RHRcf4QbegIHRlADmfovJQ7SpnsJXuMzhhnoqNCbNCPjy6WUMM3h8M8LzFZV52pIUWcPM7ahlTT92BCXbH21WUckg3REeCA663L-rBin3K7rzkFi42OplFLCXGHf_0k12z7pQEmHH2v3m-x1bPetd8i3q_z4TaZtbryBD3yel_c0_t6gT6_ef3p6prcfHj77mp7Q0zHaSXc6tEAH1ybo6em76XUnHnmB8kZdTAJmJy0PYXJQxuy52BGZs3gp8FoyfgFenHyHnL6urhS1RyKcfu9ji4tRQHvZCf7dv4DZd3YD0IcrXBCTU6lZOfVIYdZ51UBVcek1Ckp1ZJSx6QUbT3P7_XLNDv7t-NPNA1gJ6C0p7hzWd2lJce2nX9YfwNp058p</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Doai, Mariko</creator><creator>Watanabe, Naoto</creator><creator>Takahashi, Tomoko</creator><creator>Taniguchi, Mitsuru</creator><creator>Tonami, Hisao</creator><creator>Iwabuchi, Kuniyoshi</creator><creator>Kayano, Daiki</creator><creator>Fukuoka, Makoto</creator><creator>Kinuya, Seigo</creator><general>Springer Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20130401</creationdate><title>Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes</title><author>Doai, Mariko ; Watanabe, Naoto ; Takahashi, Tomoko ; Taniguchi, Mitsuru ; Tonami, Hisao ; Iwabuchi, Kuniyoshi ; Kayano, Daiki ; Fukuoka, Makoto ; Kinuya, Seigo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-3da9c137e85850c5588a32f2f78320e1b61be8d501bf1232531c92dc7fb7ca823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>DNA Damage</topic><topic>Female</topic><topic>Histones - genetics</topic><topic>Humans</topic><topic>Imaging</topic><topic>Iodine Radioisotopes - adverse effects</topic><topic>Iodine Radioisotopes - therapeutic use</topic><topic>Lymphocytes - metabolism</topic><topic>Lymphocytes - radiation effects</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Nuclear Medicine</topic><topic>Original Article</topic><topic>Radiation Dosage</topic><topic>Radiology</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - radiotherapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doai, Mariko</creatorcontrib><creatorcontrib>Watanabe, Naoto</creatorcontrib><creatorcontrib>Takahashi, Tomoko</creatorcontrib><creatorcontrib>Taniguchi, Mitsuru</creatorcontrib><creatorcontrib>Tonami, Hisao</creatorcontrib><creatorcontrib>Iwabuchi, Kuniyoshi</creatorcontrib><creatorcontrib>Kayano, Daiki</creatorcontrib><creatorcontrib>Fukuoka, Makoto</creatorcontrib><creatorcontrib>Kinuya, Seigo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Annals of nuclear medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doai, Mariko</au><au>Watanabe, Naoto</au><au>Takahashi, Tomoko</au><au>Taniguchi, Mitsuru</au><au>Tonami, Hisao</au><au>Iwabuchi, Kuniyoshi</au><au>Kayano, Daiki</au><au>Fukuoka, Makoto</au><au>Kinuya, Seigo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes</atitle><jtitle>Annals of nuclear medicine</jtitle><stitle>Ann Nucl Med</stitle><addtitle>Ann Nucl Med</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>27</volume><issue>3</issue><spage>233</spage><epage>238</epage><pages>233-238</pages><issn>0914-7187</issn><eissn>1864-6433</eissn><abstract>Purpose
The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection.
Methods
This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining.
Results
The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant (
P
= 0.001 < 0.05). Absorbed doses estimated for the 15 patients were 0.77 ± 0.31 Gy applying standard line in vitro external radiation doses.
Conclusion
γ-H2AX foci immunodetection in lymphocytes may detect radiation-induced DNA damage associated with I-131 therapy for thyroid cancer, and may facilitate estimation of the radiation doses absorbed with this therapy.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>23264066</pmid><doi>10.1007/s12149-012-0678-0</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult DNA Damage Female Histones - genetics Humans Imaging Iodine Radioisotopes - adverse effects Iodine Radioisotopes - therapeutic use Lymphocytes - metabolism Lymphocytes - radiation effects Male Medicine Medicine & Public Health Middle Aged Nuclear Medicine Original Article Radiation Dosage Radiology Thyroid Neoplasms - genetics Thyroid Neoplasms - radiotherapy |
| title | Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes |
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