The effect of rosmarinic acid on 1,2-dimethylhydrazine induced colon carcinogenesis

This study was carried out to investigate the chemopreventive potential of rosmarinic acid (RA) against 1,2-dimethylhydrazine (DMH) induced rat colon carcinogenesis by evaluating the effect of RA on tumour formation, antioxidant enzymes, cytochrome P450 content, p-nitrophenol hydroxylase and GST act...

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Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2013-05, Vol.65 (4), p.409-418
Main Authors: Venkatachalam, Karthikkumar, Gunasekaran, Sivagami, Jesudoss, Victor Antony Santiago, Namasivayam, Nalini
Format: Article
Language:English
Subjects:
1,2-Dimethylhydrazine
1,2-Dimethylhydrazine - toxicity
Animals
Antineoplastic Agents - pharmacology
antioxidants
Antioxidants - pharmacology
carcinogenesis
Carcinogens - toxicity
Chemoprevention
Cinnamates - pharmacology
colon
Colonic Neoplasms - chemically induced
Colonic Neoplasms - prevention & control
colorectal neoplasms
cytochrome P-450
Depsides - pharmacology
diet
Dietary Supplements
Disease Models, Animal
enzymes
lipid peroxidation
liver
Male
oxidative stress
Oxidative Stress - drug effects
p-nitrophenol
Rats
Rats, Wistar
Rosmarinic Acid
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Summary:This study was carried out to investigate the chemopreventive potential of rosmarinic acid (RA) against 1,2-dimethylhydrazine (DMH) induced rat colon carcinogenesis by evaluating the effect of RA on tumour formation, antioxidant enzymes, cytochrome P450 content, p-nitrophenol hydroxylase and GST activities. Rats were divided into six groups and fed modified pellet diet for the entire experimental period. Group 1 served as control, group 2 received RA (10mg/kgb.w.). Groups 3–6 were induced colon cancer by injecting DMH (20mg/kgb.w.) subcutaneously once a week for the first four weeks (groups 3–6). In addition, RA was administered at the doses of 2.5, 5 and 10mg/kgb.w. to groups 4–6 respectively. DMH treated rats showed large number of colonic tumours; decreased lipid peroxidation; decreased antioxidant status; elevated CYP450 content and PNPH activities; and decreased GST activity in the liver and colon. Supplementation with RA (5mgkg/b.w.) to DMH treated rats significantly decreased the number of polyps (50%); reversed the markers of oxidative stress (21.0%); antioxidant status (38.55%); CYP450 content (29.41%); and PNPH activities (21.9%). RA at the dose of 5mg/kgb.w. showed a most pronounced effect and could be used as a possible chemopreventive agent against colon cancer.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2011.12.005