The effects of epidermal growth factor (EGF) on the in vitro development of isolated goat secondary follicles and the relative mRNA expression of EGF, EGF-R, FSH-R and P450 aromatase in cultured follicles

The effects of varying concentrations of EGF were evaluated in terms of in vitro follicular development and the mRNA expression levels of EGF, EGF-R, FSH-R and P450 aromatase. After 6days, the addition of 50ng/mL of EGF to the culture medium increased the antrum formation rates in comparison to cult...

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Bibliographic Details
Published in:Research in veterinary science 2013-06, Vol.94 (3), p.453-461
Main Authors: Silva, C.M.G., Castro, S.V., Faustino, L.R., Rodrigues, G.Q., Brito, I.R., Rossetto, R., Saraiva, M.V.A., Campello, C.C., Lobo, C.H., Souza, C.E.A., Moura, A.A.A., Donato, M.A.M., Peixoto, C.A., Figueiredo, J.R.
Format: Article
Language:English
Subjects:
Animals
Aromatase - biosynthesis
Cells
Chromatin - metabolism
culture media
EGF
Epidermal growth factor
Epidermal Growth Factor - biosynthesis
Epidermal Growth Factor - pharmacology
estradiol
Estradiol - analysis
Estradiol - secretion
Female
follicular development
gene expression
Goat
Goats
In vitro development
In Vitro Techniques
Kinases
meiosis
messenger RNA
Mineral oils
oocytes
Oocytes - metabolism
Ovarian Follicle - chemistry
Ovarian Follicle - drug effects
Ovarian Follicle - metabolism
Ovarian Follicle - physiology
Ovarian Follicle - ultrastructure
Proteins
Receptor, Epidermal Growth Factor - biosynthesis
Receptors, FSH - biosynthesis
RNA, Messenger - metabolism
Rodents
Secondary follicle
secretion
Studies
unspecific monooxygenase
Veterinary medicine
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Summary:The effects of varying concentrations of EGF were evaluated in terms of in vitro follicular development and the mRNA expression levels of EGF, EGF-R, FSH-R and P450 aromatase. After 6days, the addition of 50ng/mL of EGF to the culture medium increased the antrum formation rates in comparison to cultured control and after 18days of culture produced oocytes with higher rates of meiosis resumption when compared to the other treatments (P
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2012.12.002