Quantification of TRPV1 Protein Levels in Rat Tissues to Understand its Physiological Roles

• Published in: Journal of molecular neuroscience 2013-05, Vol.50 (1), p.23-32
• Main Authors: Han, Ping, Korepanova, Alla V., Vos, Melissa H., Moreland, Robert B., Chiu, Mark L., Faltynek, Connie R.
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Brain - metabolism; Brain research; Cell Biology; Diabetic neuropathy; Enzymes; HEK293 Cells; Humans; Laboratories; Male; Monoclonal antibodies; Nervous system; Neurochemistry; Neurology; Neurosciences; Nociceptive Pain - chemically induced; Nociceptive Pain - metabolism; Organ Specificity; Osteoarthritis - chemically induced; Osteoarthritis - metabolism; Pain; Peripheral Nervous System - metabolism; Physiology; Proteins; Proteomics; R&D; Rats; Rats, Sprague-Dawley; Research & development; Spinal cord; Spinal Cord - metabolism; TRPV Cation Channels - genetics; TRPV Cation Channels - metabolism
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-012-9849-7

Transient receptor potential subfamily V, member 1 (TRPV1) is a nonselective cation channel expressed in both the peripheral and central nervous systems (CNS). TRPV1 protein levels in rat tissues were determined under normal and pain states using enzyme-linked immunosorbent assay. In naive rats, brain TRPV1 protein concentrations ranged from 1.5 to 4 ng/mg in hippocampus, cortex, hypothalamus, and cerebellum. Rat spinal cord TRPV1 protein levels were 40–50 ng/mg in L1–L5 of the lumbar regions, but increased to 97 ± 9.3 ng/mg toward the end of the lumbar region (L6–S1). In the complete Freund’s adjuvant (CFA)-induced inflammatory pain model, TRPV1 protein level significantly increased on both the contralateral (36.5 %, p