Electrolytic lesion of the nucleus incertus retards extinction of auditory conditioned fear

• Published in: Behavioural brain research 2013-06, Vol.247, p.201-210
• Main Authors: Pereira, C.W., Santos, F.N., Sánchez-Pérez, A.M., Otero-García, M., Marchioro, M., Ma, S., Gundlach, A.L., Olucha-Bordonau, F.E.
• Format: Article
• Language: English
• Subjects: Acoustic Stimulation; Amygdala; Amygdala - physiology; Animals; Behavior, Animal - physiology; Behavioral psychophysiology; Biological and medical sciences; Calbindin 2; Conditioning, Classical - physiology; Electrolytic lesion; Extinction; Extinction, Psychological - physiology; Fear - physiology; Fear memory; Fundamental and applied biological sciences. Psychology; Male; Nerve Tissue Proteins - metabolism; Neural Pathways - physiology; Neurons - physiology; Nucleus incertus; Pons - metabolism; Pons - physiology; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rats; Rats, Sprague-Dawley; Relaxin - metabolism; Relaxin-3; S100 Calcium Binding Protein G - metabolism
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2013.03.025

•The pontine n. incertus role in fear conditioning and extinction has been studied.•An important projection from the n. incertus to amygdala nuclei has been demonstrated.•Lesions of the n. incertus do not affect the acquisition or retrieval of fear to cue or context.•Lesions of the n. incertus delay the within trial extinction.•Thus n. incertus facilitates extinction. Fear memory circuits in the brain function to allow animals and humans to recognize putative sources of danger and adopt an appropriate behavioral response; and research on animal models of fear have helped reveal the anatomical and neurochemical nature of these circuits. The nucleus (n.) incertus in the dorsal pontine tegmentum provides a strong GABAergic projection to forebrain ‘fear centers’ and is strongly activated by neurogenic stressors. In this study in adult male rats, we examined the effect of electrolytic lesions of n. incertus on different stages of the fear conditioning-extinction process and correlated the outcomes with anatomical data on the distribution of n. incertus-derived nerve fibers in areas implicated in fear circuits. In a contextual auditory fear conditioning paradigm, we compared freezing behavior in control (naïve) rats (n=23) and rats with sham- or electrolytic lesions of n. incertus (n=13/group). The effectiveness and extent of the lesions was assessed post-mortem using immunohistochemical markers for n. incertus neurons-calretinin and relaxin-3. There were no differences between the three experimental groups in the habituation, acquisition, or context conditioning phases; but n. incertus lesioned rats displayed a markedly slower, ‘delayed’ extinction of conditioned freezing responses compared to sham-lesion and control rats, but no differences in retrieval of extinguished fear. These and earlier findings suggest that n. incertus-related circuits normally promote extinction through inhibitory projections to the amygdala, which is involved in acquisition of extinction memories.