The clearance time of infused hematopoietic stem cell from the blood circulation

• Published in: Transfusion and apheresis science 2013-04, Vol.48 (2), p.235-239
• Main Authors: Donmez, Ayhan, Ozsan, Fatma, Arik, Bahar, Ozkayin, Nese, Çagirgan, Seckin, Mir, Sevgi, Vural, Filiz, Tombuloglu, Murat
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antigens, CD34 - blood; CD34+ cell levels; Engraftment; Female; Flow Cytometry; Graft Survival; Health technology assessment; Hematology, Oncology and Palliative Medicine; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Hodgkin Disease - blood; Hodgkin Disease - therapy; Homing; Humans; Leukocyte Count; Male; Middle Aged; Multiple Myeloma - blood; Multiple Myeloma - therapy; Peripheral Blood Stem Cell Transplantation; Platelet Count; Prospective Studies; Stem cell transplantation; Time Factors; Transplantation, Autologous
• ISSN: 1473-0502; 1878-1683
• DOI: 10.1016/j.transci.2013.01.005

Abstract There is no detailed information about the clearance time of infused hematopoietic stem cell (HSC) from the blood circulation in humans. In this prospective study, peripheral blood CD34+ cell counts were detected during the 4 days period following autologous HSC transplantation in 20 patients by means of flow cytometry. The median CD34+ cells were at the highest level in the first hour and decreased below pre-infusion values on the first day after HSC infusion. By nonparametric analysis, positive correlation was found between CD34+ cell levels at the first hour and the post-thaw CD34+ cell dose ( r = 0.57, p = 0.01). An inverse correlation was determined between CD34+ cell levels at the first hour and neutrophil engraftment ( r = −0.54, p = 0.01). Compared with the patients having CD34+ cell count of ⩾2 μL−1 in the first hour following HSC infusion, the patients having CD34+ cell count of