Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats

Objective To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats. Methods Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by s...

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Bibliographic Details
Published in:Chinese journal of integrative medicine 2013-05, Vol.19 (5), p.367-373
Main Authors: Luo, Jie-kun, Zhou, Hua-jun, Wu, Jing, Tang, Tao, Liang, Qing-hua
Format: Article
Language:English
Subjects:
Acupuncture Points
Animals
Brain - metabolism
Brain - pathology
Cell Proliferation
Cerebral Hemorrhage - genetics
Cerebral Hemorrhage - pathology
Cerebral Hemorrhage - physiopathology
Cerebral Hemorrhage - therapy
Electroacupuncture
Endothelial Cells - pathology
Gene Expression Regulation
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Immunohistochemistry
Male
Medicine
Medicine & Public Health
Neovascularization, Physiologic
Original Article
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:Objective To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats. Methods Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these, one group was not further treated, the second group underwent Zusanli (ST36)-acupuncture, and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations. Acupuncture was performed by stimulation with electrical needles at frequencies of 2–20 Hz for 30 min per day. Angiogenesis on days 3, 7 and 14 was assessed by double immunolabeling, and expression of HIF-1α was evaluated by immunohistochemistry, quantitative real time reverse transcription-polymerase chain reaction and Western blotting. Results 5-Bromo-2-deoxyuridine (BrdU)-labeled nuclei in cerebral endothelial cells (ECs) resided around the hematoma and the labeling peaked from 7 to 14 days ( P
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-013-1458-y