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Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats
Objective To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats. Methods Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by s...
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| Published in: | Chinese journal of integrative medicine 2013-05, Vol.19 (5), p.367-373 |
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| container_issue | 5 |
| container_start_page | 367 |
| container_title | Chinese journal of integrative medicine |
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| creator | Luo, Jie-kun Zhou, Hua-jun Wu, Jing Tang, Tao Liang, Qing-hua |
| description | Objective
To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats.
Methods
Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these, one group was not further treated, the second group underwent Zusanli (ST36)-acupuncture, and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations. Acupuncture was performed by stimulation with electrical needles at frequencies of 2–20 Hz for 30 min per day. Angiogenesis on days 3, 7 and 14 was assessed by double immunolabeling, and expression of HIF-1α was evaluated by immunohistochemistry, quantitative real time reverse transcription-polymerase chain reaction and Western blotting.
Results
5-Bromo-2-deoxyuridine (BrdU)-labeled nuclei in cerebral endothelial cells (ECs) resided around the hematoma and the labeling peaked from 7 to 14 days (
P |
| doi_str_mv | 10.1007/s11655-013-1458-y |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1352278404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1352278404</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-1dc2b1cc2772ae907654744ed6713408891c9120b58a31e544ace332982d79c53</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EoqXwA1hQxjIEfP6IkxFV5UOqxEBZWCzXubapUqfY8dB_j6sWRiaf5Od9dfcQcgv0AShVjwGgkDKnwHMQssz3Z2QIVcVzKig7T3OhWJpBDshVCBtKpSqovCQDxgslANiQLKct2t53xsZddLaPHjPTZ18xGNc22fhjzov7zFiLLXrTY8ga13tj0ePCmzZb47bzfm1WmDeujhbrzLhV063QYWgOdJZi4ZpcLE0b8Ob0jsjn83Q-ec1n7y9vk6dZbrkQfQ61ZQuwlinFDFZUFVIoIbAuFHBBy7ICWwGjC1kaDiiFSJtwzqqS1aqyko_I-Ni78913xNDrbRPS7q1x2MWggUvGVCmoSCgcUeu7EDwu9c43W-P3Gqg-6NVHvTrp1Qe9ep8yd6f6uNhi_Zf49ZkAdgRC-nIr9HrTRe_Syf-0_gDHSYXd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1352278404</pqid></control><display><type>article</type><title>Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats</title><source>Springer Nature</source><creator>Luo, Jie-kun ; Zhou, Hua-jun ; Wu, Jing ; Tang, Tao ; Liang, Qing-hua</creator><creatorcontrib>Luo, Jie-kun ; Zhou, Hua-jun ; Wu, Jing ; Tang, Tao ; Liang, Qing-hua</creatorcontrib><description>Objective
To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats.
Methods
Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these, one group was not further treated, the second group underwent Zusanli (ST36)-acupuncture, and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations. Acupuncture was performed by stimulation with electrical needles at frequencies of 2–20 Hz for 30 min per day. Angiogenesis on days 3, 7 and 14 was assessed by double immunolabeling, and expression of HIF-1α was evaluated by immunohistochemistry, quantitative real time reverse transcription-polymerase chain reaction and Western blotting.
Results
5-Bromo-2-deoxyuridine (BrdU)-labeled nuclei in cerebral endothelial cells (ECs) resided around the hematoma and the labeling peaked from 7 to 14 days (
P
<0.01). HIF-1α positive microvessels with a dilated outline were detected in perihematomal tissues after ICH, with the vessels extending into the clot from the surrounding area beginning on day 7. Following ICH, HIF-1α protein levels increased (
P
<0.05), but HIF-1α mRNA levels did not change. Electro-acupuncture at the Zusanli (ST36) acupoint increased BrdU-labeled nuclei in cerebral ECs (
P
<0.05) and up-regulated the expression of HIF-1α protein (
P
<0.05), but had little effect on the spatial distribution of HIF-1α or on HIF-1α mRNA levels.
Conclusions
: Electro-acupuncture treatment at the Zusanli (ST36) acupoint may accelerate ICH-induced angiogenesis by up-regulating HIF-1α protein, and may enhance recovery following hemorrhagic cerebral injury.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-013-1458-y</identifier><identifier>PMID: 23674112</identifier><language>eng</language><publisher>Heidelberg: Chinese Association of Traditional and Western Medicine</publisher><subject>Acupuncture Points ; Animals ; Brain - metabolism ; Brain - pathology ; Cell Proliferation ; Cerebral Hemorrhage - genetics ; Cerebral Hemorrhage - pathology ; Cerebral Hemorrhage - physiopathology ; Cerebral Hemorrhage - therapy ; Electroacupuncture ; Endothelial Cells - pathology ; Gene Expression Regulation ; Hypoxia-Inducible Factor 1, alpha Subunit - genetics ; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism ; Immunohistochemistry ; Male ; Medicine ; Medicine & Public Health ; Neovascularization, Physiologic ; Original Article ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Chinese journal of integrative medicine, 2013-05, Vol.19 (5), p.367-373</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-1dc2b1cc2772ae907654744ed6713408891c9120b58a31e544ace332982d79c53</citedby><cites>FETCH-LOGICAL-c344t-1dc2b1cc2772ae907654744ed6713408891c9120b58a31e544ace332982d79c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23674112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Jie-kun</creatorcontrib><creatorcontrib>Zhou, Hua-jun</creatorcontrib><creatorcontrib>Wu, Jing</creatorcontrib><creatorcontrib>Tang, Tao</creatorcontrib><creatorcontrib>Liang, Qing-hua</creatorcontrib><title>Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats.
Methods
Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these, one group was not further treated, the second group underwent Zusanli (ST36)-acupuncture, and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations. Acupuncture was performed by stimulation with electrical needles at frequencies of 2–20 Hz for 30 min per day. Angiogenesis on days 3, 7 and 14 was assessed by double immunolabeling, and expression of HIF-1α was evaluated by immunohistochemistry, quantitative real time reverse transcription-polymerase chain reaction and Western blotting.
Results
5-Bromo-2-deoxyuridine (BrdU)-labeled nuclei in cerebral endothelial cells (ECs) resided around the hematoma and the labeling peaked from 7 to 14 days (
P
<0.01). HIF-1α positive microvessels with a dilated outline were detected in perihematomal tissues after ICH, with the vessels extending into the clot from the surrounding area beginning on day 7. Following ICH, HIF-1α protein levels increased (
P
<0.05), but HIF-1α mRNA levels did not change. Electro-acupuncture at the Zusanli (ST36) acupoint increased BrdU-labeled nuclei in cerebral ECs (
P
<0.05) and up-regulated the expression of HIF-1α protein (
P
<0.05), but had little effect on the spatial distribution of HIF-1α or on HIF-1α mRNA levels.
Conclusions
: Electro-acupuncture treatment at the Zusanli (ST36) acupoint may accelerate ICH-induced angiogenesis by up-regulating HIF-1α protein, and may enhance recovery following hemorrhagic cerebral injury.</description><subject>Acupuncture Points</subject><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Brain - pathology</subject><subject>Cell Proliferation</subject><subject>Cerebral Hemorrhage - genetics</subject><subject>Cerebral Hemorrhage - pathology</subject><subject>Cerebral Hemorrhage - physiopathology</subject><subject>Cerebral Hemorrhage - therapy</subject><subject>Electroacupuncture</subject><subject>Endothelial Cells - pathology</subject><subject>Gene Expression Regulation</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neovascularization, Physiologic</subject><subject>Original Article</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EoqXwA1hQxjIEfP6IkxFV5UOqxEBZWCzXubapUqfY8dB_j6sWRiaf5Od9dfcQcgv0AShVjwGgkDKnwHMQssz3Z2QIVcVzKig7T3OhWJpBDshVCBtKpSqovCQDxgslANiQLKct2t53xsZddLaPHjPTZ18xGNc22fhjzov7zFiLLXrTY8ga13tj0ePCmzZb47bzfm1WmDeujhbrzLhV063QYWgOdJZi4ZpcLE0b8Ob0jsjn83Q-ec1n7y9vk6dZbrkQfQ61ZQuwlinFDFZUFVIoIbAuFHBBy7ICWwGjC1kaDiiFSJtwzqqS1aqyko_I-Ni78913xNDrbRPS7q1x2MWggUvGVCmoSCgcUeu7EDwu9c43W-P3Gqg-6NVHvTrp1Qe9ep8yd6f6uNhi_Zf49ZkAdgRC-nIr9HrTRe_Syf-0_gDHSYXd</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Luo, Jie-kun</creator><creator>Zhou, Hua-jun</creator><creator>Wu, Jing</creator><creator>Tang, Tao</creator><creator>Liang, Qing-hua</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats</title><author>Luo, Jie-kun ; Zhou, Hua-jun ; Wu, Jing ; Tang, Tao ; Liang, Qing-hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-1dc2b1cc2772ae907654744ed6713408891c9120b58a31e544ace332982d79c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acupuncture Points</topic><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Brain - pathology</topic><topic>Cell Proliferation</topic><topic>Cerebral Hemorrhage - genetics</topic><topic>Cerebral Hemorrhage - pathology</topic><topic>Cerebral Hemorrhage - physiopathology</topic><topic>Cerebral Hemorrhage - therapy</topic><topic>Electroacupuncture</topic><topic>Endothelial Cells - pathology</topic><topic>Gene Expression Regulation</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neovascularization, Physiologic</topic><topic>Original Article</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Jie-kun</creatorcontrib><creatorcontrib>Zhou, Hua-jun</creatorcontrib><creatorcontrib>Wu, Jing</creatorcontrib><creatorcontrib>Tang, Tao</creatorcontrib><creatorcontrib>Liang, Qing-hua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Jie-kun</au><au>Zhou, Hua-jun</au><au>Wu, Jing</au><au>Tang, Tao</au><au>Liang, Qing-hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>19</volume><issue>5</issue><spage>367</spage><epage>373</epage><pages>367-373</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats.
Methods
Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these, one group was not further treated, the second group underwent Zusanli (ST36)-acupuncture, and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations. Acupuncture was performed by stimulation with electrical needles at frequencies of 2–20 Hz for 30 min per day. Angiogenesis on days 3, 7 and 14 was assessed by double immunolabeling, and expression of HIF-1α was evaluated by immunohistochemistry, quantitative real time reverse transcription-polymerase chain reaction and Western blotting.
Results
5-Bromo-2-deoxyuridine (BrdU)-labeled nuclei in cerebral endothelial cells (ECs) resided around the hematoma and the labeling peaked from 7 to 14 days (
P
<0.01). HIF-1α positive microvessels with a dilated outline were detected in perihematomal tissues after ICH, with the vessels extending into the clot from the surrounding area beginning on day 7. Following ICH, HIF-1α protein levels increased (
P
<0.05), but HIF-1α mRNA levels did not change. Electro-acupuncture at the Zusanli (ST36) acupoint increased BrdU-labeled nuclei in cerebral ECs (
P
<0.05) and up-regulated the expression of HIF-1α protein (
P
<0.05), but had little effect on the spatial distribution of HIF-1α or on HIF-1α mRNA levels.
Conclusions
: Electro-acupuncture treatment at the Zusanli (ST36) acupoint may accelerate ICH-induced angiogenesis by up-regulating HIF-1α protein, and may enhance recovery following hemorrhagic cerebral injury.</abstract><cop>Heidelberg</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>23674112</pmid><doi>10.1007/s11655-013-1458-y</doi><tpages>7</tpages></addata></record> |
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| ispartof | Chinese journal of integrative medicine, 2013-05, Vol.19 (5), p.367-373 |
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| source | Springer Nature |
| subjects | Acupuncture Points Animals Brain - metabolism Brain - pathology Cell Proliferation Cerebral Hemorrhage - genetics Cerebral Hemorrhage - pathology Cerebral Hemorrhage - physiopathology Cerebral Hemorrhage - therapy Electroacupuncture Endothelial Cells - pathology Gene Expression Regulation Hypoxia-Inducible Factor 1, alpha Subunit - genetics Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Immunohistochemistry Male Medicine Medicine & Public Health Neovascularization, Physiologic Original Article Rats Rats, Sprague-Dawley RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats |
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