Metabolic Profiling of Phenolic Acids and Oxidative Stress Markers after Consumption of Lonicera caerulea L. Fruit

• Published in: Journal of agricultural and food chemistry 2013-05, Vol.61 (19), p.4526-4532
• Main Authors: Heinrich, Jan, Valentová, Kateřina, Vacek, Jan, Palíková, Irena, Zatloukalová, Martina, Kosina, Pavel, Ulrichová, Jitka, Vrbková, Jana, Šimánek, Vilím
• Format: Article
• Language: English
• Subjects: Adult; anthocyanins; Anthocyanins - administration & dosage; Antioxidants - metabolism; Benzoic Acid - urine; Biological and medical sciences; Biomarkers - urine; catalase; Catalase - blood; Chromatography, Liquid; Cinnamates - urine; Coumaric Acids - urine; creatinine; erythrocytes; Erythrocytes - metabolism; excretion; Female; Food industries; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; glutathione peroxidase; Glutathione Peroxidase - blood; Hippurates - urine; Humans; Hydroxybenzoates - urine; ingestion; Lipoproteins, LDL - blood; Lonicera - chemistry; Lonicera caerulea; low density lipoprotein; Male; Mass Spectrometry; Metabolome; metabolomics; oxidation; oxidative stress; Oxidative Stress - drug effects; phenolic acids; protein products; small fruits; thiobarbituric acid; Thiobarbituric Acid Reactive Substances - metabolism; urine; Vanillic Acid - urine; volunteers
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf304150b

This study investigated the effect of one-week consumption of 165 g/day fresh blue honeysuckle berries (208 mg/day anthocyanins) in 10 healthy volunteers. At the end of intervention, levels of benzoic (median 1782 vs 4156), protocatechuic (709 vs 2417), vanillic (2779 vs 4753), 3-hydroxycinnamic (143 vs 351), p-coumaric (182 vs 271), isoferulic (805 vs 1570), ferulic (1086 vs 2395), and hippuric (194833 vs 398711 μg/mg creatinine) acids by LC/MS were significantly increased in the urine. Clinical chemistry safety markers were not altered. Oxidative stress markers, erythrocyte glutathione peroxidase (0.73 vs 0.88 U/g Hb) and catalase (2.5 vs 2.8 μkat/g Hb) activities, and erythrocyte/plasma thiobarbituric acid reactive substance (522 vs 612/33 vs 38 μmol/g Hb/protein) levels were significantly increased, without change in plasma antioxidant status. Nonsignificant changes of advanced oxidation protein products and oxidized LDL were observed. The results provide a solid base for further study of metabolite excretion and antioxidant parameters after ingestion of anthocyanins.