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Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita
In this study, the 3,4-dihydroxybenzoic acid (3,4-DHBA) from Terminalia nigrovenulosa bark (TNB) was purified and its in vitro nematicidal activity was investigated against Meloidogyne incognita. The purification of 3,4-DHBA used a silica gel column and Sephadex LH-20 chromatography combined with th...
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| Published in: | Microbial pathogenesis 2013-06, Vol.59-60, p.52-59 |
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| container_end_page | 59 |
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| container_start_page | 52 |
| container_title | Microbial pathogenesis |
| container_volume | 59-60 |
| creator | Nguyen, Dang-Minh-Chanh Seo, Dong-Jun Kim, Kil-Yong Park, Ro-Dong Kim, Dong-Hyun Han, Yeon-Soo Kim, Tae-Hwan Jung, Woo-Jin |
| description | In this study, the 3,4-dihydroxybenzoic acid (3,4-DHBA) from Terminalia nigrovenulosa bark (TNB) was purified and its in vitro nematicidal activity was investigated against Meloidogyne incognita. The purification of 3,4-DHBA used a silica gel column and Sephadex LH-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. Structural identification of the 3,4-DHBA was conducted using 1H nuclear magnetic resonance (NMR), 13C NMR, and liquid chromatography time-of-flight mass spectrometry. Nematicidal activity bioassays revealed that 3,4-DHBA treatment resulted in 33.3, 47.5, 72.5 and 94.2% J2 mortality at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively after 12 h incubation. J2 mortality was increased significantly (P |
| doi_str_mv | 10.1016/j.micpath.2013.04.005 |
| format | article |
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•Terminalia nigrovenulosa extracts showed high nematicidal activity against Meloidogyne incognita.•Ethyl acetate fraction of TNB demonstrated the highest nematicidal activity.•3,4-DHBA purified from TNB possess the high nematicidal activity against M. incognita.•Changes in the shape of the eggs were determined after incubation with a 3,4-DHBA.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2013.04.005</identifier><identifier>PMID: 23603737</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Anthelmintics - isolation & purification ; Anthelmintics - pharmacology ; bark ; bioassays ; Biological Assay ; Chromatography, Gel ; Chromatography, High Pressure Liquid ; Chromatography, Thin Layer ; Egg morphology ; eggs ; Fluorescence staining ; high performance liquid chromatography ; Hydroxybenzoates - isolation & purification ; Hydroxybenzoates - pharmacology ; Magnetic Resonance Spectroscopy ; mass spectrometry ; Meloidogyne incognita ; mortality ; Nematicidal compound ; nematicidal properties ; nuclear magnetic resonance spectroscopy ; Plant Bark - chemistry ; Plant extract ; silica gel ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Survival Analysis ; Terminalia ; Terminalia - chemistry ; thin layer chromatography ; Tylenchoidea - drug effects ; Tylenchoidea - physiology ; Zygote - drug effects</subject><ispartof>Microbial pathogenesis, 2013-06, Vol.59-60, p.52-59</ispartof><rights>2013 The authors</rights><rights>Copyright © 2013 The authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-58f32d8ea66f49e30cc8b12fc56660320ce9eda5364265a97553e04d600bcfe93</citedby><cites>FETCH-LOGICAL-c502t-58f32d8ea66f49e30cc8b12fc56660320ce9eda5364265a97553e04d600bcfe93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23603737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Dang-Minh-Chanh</creatorcontrib><creatorcontrib>Seo, Dong-Jun</creatorcontrib><creatorcontrib>Kim, Kil-Yong</creatorcontrib><creatorcontrib>Park, Ro-Dong</creatorcontrib><creatorcontrib>Kim, Dong-Hyun</creatorcontrib><creatorcontrib>Han, Yeon-Soo</creatorcontrib><creatorcontrib>Kim, Tae-Hwan</creatorcontrib><creatorcontrib>Jung, Woo-Jin</creatorcontrib><title>Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>In this study, the 3,4-dihydroxybenzoic acid (3,4-DHBA) from Terminalia nigrovenulosa bark (TNB) was purified and its in vitro nematicidal activity was investigated against Meloidogyne incognita. The purification of 3,4-DHBA used a silica gel column and Sephadex LH-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. Structural identification of the 3,4-DHBA was conducted using 1H nuclear magnetic resonance (NMR), 13C NMR, and liquid chromatography time-of-flight mass spectrometry. Nematicidal activity bioassays revealed that 3,4-DHBA treatment resulted in 33.3, 47.5, 72.5 and 94.2% J2 mortality at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively after 12 h incubation. J2 mortality was increased significantly (P < 0.0001) with increasing incubation time in the range of 54.2–94.2% from 3 to 9 h after incubation with 3,4-DHBA (1.0 mg/ml), but with no significant difference observed where the incubation time was increased from 9 to 12 h. The 3,4-DHBA treatment resulted in 33.3, 65.0, 76.7 and 85.0% hatch inhibition at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively, 3 days after incubation. Changes in the shape of the eggs were determined after incubation for 1 day with a 3,4-DHBA concentration of 1.0 mg/ml.
•Terminalia nigrovenulosa extracts showed high nematicidal activity against Meloidogyne incognita.•Ethyl acetate fraction of TNB demonstrated the highest nematicidal activity.•3,4-DHBA purified from TNB possess the high nematicidal activity against M. incognita.•Changes in the shape of the eggs were determined after incubation with a 3,4-DHBA.</description><subject>Animals</subject><subject>Anthelmintics - isolation & purification</subject><subject>Anthelmintics - pharmacology</subject><subject>bark</subject><subject>bioassays</subject><subject>Biological Assay</subject><subject>Chromatography, Gel</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Thin Layer</subject><subject>Egg morphology</subject><subject>eggs</subject><subject>Fluorescence staining</subject><subject>high performance liquid chromatography</subject><subject>Hydroxybenzoates - isolation & purification</subject><subject>Hydroxybenzoates - pharmacology</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>mass spectrometry</subject><subject>Meloidogyne incognita</subject><subject>mortality</subject><subject>Nematicidal compound</subject><subject>nematicidal properties</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>Plant Bark - chemistry</subject><subject>Plant extract</subject><subject>silica gel</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Survival Analysis</subject><subject>Terminalia</subject><subject>Terminalia - chemistry</subject><subject>thin layer chromatography</subject><subject>Tylenchoidea - drug effects</subject><subject>Tylenchoidea - physiology</subject><subject>Zygote - drug effects</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkMFu1DAQhi0EotvCIwA-ciBhbMfZ5ISqCgpSgQPt2XLsSTpLEi92siLw8qTahSunuXzzzz8fYy8E5AJE-XaXD-T2drrPJQiVQ5ED6EdsI6AuMyGhesw2UFUyK0DAGTtPaQcAdaHqp-xMqhLUVm037PcXHOxEjrztuXUTHWhaeGi5elNknu4XH8PPpcHxVyC3AuT5fo7UEnrexjDwW4wDjbYny0fqYjjgOPchWd7Y-J3bztKYJv4Z-0A-dMuInEYXupEm-4w9aW2f8PlpXrC7D-9vrz5mN1-vP11d3mROg5wyXbVK-gptWbZFjQqcqxohW6fLcn1DgsMavdWqLGSpbb3VWiEUvgRoXIu1umCvj7n7GH7MmCYzUHLY93bEMCcjlJayElqqFdVH1MWQUsTW7CMNNi5GgHnwbnbm5N08eDdQmNX7uvfydGJuBvT_tv6KXoFXR6C1wdguUjJ339aEtaSQWm7FSrw7EriqOBBGkxzh6NBTRDcZH-g_Jf4AJtGh5A</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Nguyen, Dang-Minh-Chanh</creator><creator>Seo, Dong-Jun</creator><creator>Kim, Kil-Yong</creator><creator>Park, Ro-Dong</creator><creator>Kim, Dong-Hyun</creator><creator>Han, Yeon-Soo</creator><creator>Kim, Tae-Hwan</creator><creator>Jung, Woo-Jin</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130601</creationdate><title>Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita</title><author>Nguyen, Dang-Minh-Chanh ; Seo, Dong-Jun ; Kim, Kil-Yong ; Park, Ro-Dong ; Kim, Dong-Hyun ; Han, Yeon-Soo ; Kim, Tae-Hwan ; Jung, Woo-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-58f32d8ea66f49e30cc8b12fc56660320ce9eda5364265a97553e04d600bcfe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Anthelmintics - isolation & purification</topic><topic>Anthelmintics - pharmacology</topic><topic>bark</topic><topic>bioassays</topic><topic>Biological Assay</topic><topic>Chromatography, Gel</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Thin Layer</topic><topic>Egg morphology</topic><topic>eggs</topic><topic>Fluorescence staining</topic><topic>high performance liquid chromatography</topic><topic>Hydroxybenzoates - isolation & purification</topic><topic>Hydroxybenzoates - pharmacology</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>mass spectrometry</topic><topic>Meloidogyne incognita</topic><topic>mortality</topic><topic>Nematicidal compound</topic><topic>nematicidal properties</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>Plant Bark - chemistry</topic><topic>Plant extract</topic><topic>silica gel</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Survival Analysis</topic><topic>Terminalia</topic><topic>Terminalia - chemistry</topic><topic>thin layer chromatography</topic><topic>Tylenchoidea - drug effects</topic><topic>Tylenchoidea - physiology</topic><topic>Zygote - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Dang-Minh-Chanh</creatorcontrib><creatorcontrib>Seo, Dong-Jun</creatorcontrib><creatorcontrib>Kim, Kil-Yong</creatorcontrib><creatorcontrib>Park, Ro-Dong</creatorcontrib><creatorcontrib>Kim, Dong-Hyun</creatorcontrib><creatorcontrib>Han, Yeon-Soo</creatorcontrib><creatorcontrib>Kim, Tae-Hwan</creatorcontrib><creatorcontrib>Jung, Woo-Jin</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, Dang-Minh-Chanh</au><au>Seo, Dong-Jun</au><au>Kim, Kil-Yong</au><au>Park, Ro-Dong</au><au>Kim, Dong-Hyun</au><au>Han, Yeon-Soo</au><au>Kim, Tae-Hwan</au><au>Jung, Woo-Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>59-60</volume><spage>52</spage><epage>59</epage><pages>52-59</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>In this study, the 3,4-dihydroxybenzoic acid (3,4-DHBA) from Terminalia nigrovenulosa bark (TNB) was purified and its in vitro nematicidal activity was investigated against Meloidogyne incognita. The purification of 3,4-DHBA used a silica gel column and Sephadex LH-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. Structural identification of the 3,4-DHBA was conducted using 1H nuclear magnetic resonance (NMR), 13C NMR, and liquid chromatography time-of-flight mass spectrometry. Nematicidal activity bioassays revealed that 3,4-DHBA treatment resulted in 33.3, 47.5, 72.5 and 94.2% J2 mortality at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively after 12 h incubation. J2 mortality was increased significantly (P < 0.0001) with increasing incubation time in the range of 54.2–94.2% from 3 to 9 h after incubation with 3,4-DHBA (1.0 mg/ml), but with no significant difference observed where the incubation time was increased from 9 to 12 h. The 3,4-DHBA treatment resulted in 33.3, 65.0, 76.7 and 85.0% hatch inhibition at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively, 3 days after incubation. Changes in the shape of the eggs were determined after incubation for 1 day with a 3,4-DHBA concentration of 1.0 mg/ml.
•Terminalia nigrovenulosa extracts showed high nematicidal activity against Meloidogyne incognita.•Ethyl acetate fraction of TNB demonstrated the highest nematicidal activity.•3,4-DHBA purified from TNB possess the high nematicidal activity against M. incognita.•Changes in the shape of the eggs were determined after incubation with a 3,4-DHBA.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23603737</pmid><doi>10.1016/j.micpath.2013.04.005</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anthelmintics - isolation & purification Anthelmintics - pharmacology bark bioassays Biological Assay Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Thin Layer Egg morphology eggs Fluorescence staining high performance liquid chromatography Hydroxybenzoates - isolation & purification Hydroxybenzoates - pharmacology Magnetic Resonance Spectroscopy mass spectrometry Meloidogyne incognita mortality Nematicidal compound nematicidal properties nuclear magnetic resonance spectroscopy Plant Bark - chemistry Plant extract silica gel Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Survival Analysis Terminalia Terminalia - chemistry thin layer chromatography Tylenchoidea - drug effects Tylenchoidea - physiology Zygote - drug effects |
| title | Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita |
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