Effect of bone morphogenetic protein-2, demineralized bone matrix and systemic parathyroid hormone (1-34) on local bone formation in a rat calvaria critical-size defect model

Aim To determine the potential of recombinant human bone morphogenetic protein‐2 (rhBMP‐2) soak‐loaded on to an absorbable collagen sponge (ACS) to induce local bone formation compared with the clinical reference demineralized bone matrix (DBM) and to investigate potential additive/synergistic effec...

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Published in:Journal of periodontal research 2013-04, Vol.48 (2), p.243-251
Main Authors: Stancoven, B. W., Lee, J., Dixon, D. R., McPherson III, J.C., Bisch, F. C., Wikesjö, U. M. E., Susin, C.
Format: Article
Language:English
Subjects:
Absorbable Implants
Animals
Bone Demineralization Technique
Bone Diseases - diagnostic imaging
Bone Diseases - surgery
bone formation
Bone Matrix - transplantation
Bone Morphogenetic Protein 2 - therapeutic use
Collagen
Craniotomy - methods
demineralized bone matrix
Dentistry
Drug Carriers
Drug Synergism
Humans
Male
Microscopy, Polarization
Osteogenesis - drug effects
Parathyroid Hormone - therapeutic use
PTH
Radiography
Random Allocation
Rats
Rats, Sprague-Dawley
Recombinant Proteins - therapeutic use
rhBMP-2
Skull - diagnostic imaging
Skull - drug effects
Time Factors
tissue engineering
Tissue Preservation - methods
Transforming Growth Factor beta - therapeutic use
Wound Healing - drug effects
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Summary:Aim To determine the potential of recombinant human bone morphogenetic protein‐2 (rhBMP‐2) soak‐loaded on to an absorbable collagen sponge (ACS) to induce local bone formation compared with the clinical reference demineralized bone matrix (DBM) and to investigate potential additive/synergistic effects of exogenous parathyroid hormone (PTH). Methods Critical‐size (8 mm), through‐through calvaria osteotomy defects in 160 adult male Sprague–Dawley rats were randomized to receive one of eight interventions: rhBMP‐2/ACS, DBM, ACS, or serve as controls (empty defects) combined or not with systemic PTH. Ten animals from each group were followed for 4 and 8 wks for radiographic and histometric analysis. Multivariable analysis was used to assess the effect of experimental intervention and healing time on local bone formation. Results In the multivariable analysis, rhBMP‐2/ACS exhibited significantly greater histologic bone formation than control (β ± SE: 54.76 ± 5.85, p 
ISSN:0022-3484
1600-0765
DOI:10.1111/jre.12001