Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease

Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the...

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Bibliographic Details
Published in:Molecular biotechnology 2013-06, Vol.54 (2), p.475-483
Main Authors: Larsen, Alejandra, Gonzalez, Ester Teresa, Serena, María Soledad, Echeverría, María Gabriela, Mortola, Eduardo
Format: Article
Language:English
Subjects:
Animal diseases
Animals
Antibodies, Viral - immunology
Antigens, Viral - genetics
Antigens, Viral - immunology
Antigens, Viral - metabolism
Baculoviridae - genetics
Baculoviridae - metabolism
Baculovirus
Biochemistry
Biological Techniques
Biotechnology
Bovine leukemia virus
Cattle
Cell Biology
Cell Line
Chemistry
Chemistry and Materials Science
Enzootic Bovine Leukosis - genetics
Enzootic Bovine Leukosis - metabolism
Enzootic Bovine Leukosis - virology
Glycoproteins - genetics
Glycoproteins - immunology
Glycoproteins - metabolism
Human Genetics
Immunoassay
Immunoglobulins
Insecta - genetics
Insecta - metabolism
Insecta - virology
Insects
Leukemia
Leukemia Virus, Bovine - genetics
Leukemia Virus, Bovine - metabolism
Protein Science
Proteins
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Viral Proteins - genetics
Viral Proteins - immunology
Viral Proteins - metabolism
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Summary:Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-012-9587-7