The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits

• Published in: Lasers in medical science 2013-05, Vol.28 (3), p.815-822
• Main Authors: Pinheiro, Antônio Luiz Barbosa, Santos, Nicole Ribeiro Silva, Oliveira, Priscila Chagas, Aciole, Gilberth Tadeu Santos, Ramos, Thais Andrade, Gonzalez, Tayná Assunção, da Silva, Laís Nogueira, Barbosa, Artur Felipe Santos, Silveira, Landulfo
• Format: Article
• Language: English
• Subjects: Animals; Bone Substitutes; Bone Wires; Bones; Ceramics; Dentistry; Durapatite; Fluorescence; Fracture Fixation, Internal - methods; Fractures; Guided Tissue Regeneration - methods; Infrared Rays - therapeutic use; Lasers; Lasers, Semiconductor - therapeutic use; Light therapy; Low-Level Light Therapy - methods; Male; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Photonics; Quantum Optics; Rabbits; Spectrum Analysis, Raman; Tibial Fractures - radiotherapy; Tibial Fractures - surgery
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-012-1166-4

The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm 2  = 16 J/cm 2 , ϕ  = 0.5 cm 2 , CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm 2 , 16 J/cm 2  = 112 J/cm 2 ). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases ( p