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The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits
The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm 2 = 16 J/cm 2 , ϕ = 0.5 cm 2 , CW) associated or not to the use of hydroxyapatite...
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| Published in: | Lasers in medical science 2013-05, Vol.28 (3), p.815-822 |
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| creator | Pinheiro, Antônio Luiz Barbosa Santos, Nicole Ribeiro Silva Oliveira, Priscila Chagas Aciole, Gilberth Tadeu Santos Ramos, Thais Andrade Gonzalez, Tayná Assunção da Silva, Laís Nogueira Barbosa, Artur Felipe Santos Silveira, Landulfo |
| description | The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm
2
= 16 J/cm
2
,
ϕ
= 0.5 cm
2
, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm
2
, 16 J/cm
2
= 112 J/cm
2
). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (
p
|
| doi_str_mv | 10.1007/s10103-012-1166-4 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1352294288</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1352294288</sourcerecordid><originalsourceid>FETCH-LOGICAL-c448t-3c45dc5a40f4a7c820e2d9bec2fe4f2d28955f3cf9e346176f4791b212368c123</originalsourceid><addsrcrecordid>eNqNktuK1TAUhosoznb0AbyRgDfeVHPqyTsZPAwMCMMI3pU0Xdk7Q9vUrHSkT-0ruDp7KyIIQkla1rf-fyX9s-y54K8F59UbFFxwlXMhcyHKMtcPsp3QqshLrr8-zHZclnVeN1KcZU8QbzkXVSnU4-xMylopWde77MfNARg4562xKwuOJfpeELbXy2s2GITI5kNIgQrRzCsziMF6k6BnKbDOzweD3jJL1ZH2fTQuMTP1bL_4nqAuTMAi7GEiIvkwMXpwiXuyHJiLxqYlArIU4V70u08HWiKNgAkCrhM5o8e3zDAbxtlsKndwGs0NS6BuC5OFe9drMxrSn8GmSPqYln7dHKPpOp_wafbImQHh2Wk_z758eH9z8Sm_-vzx8uLdVW61rlOurC56WxjNnTaVrSUH2TcdWOlAO9nLuikKp6xrQOmSrtXpqhGdFFKVtaX1PHt11J1j-LYApnb0NOUwmAnCgq1QhZSNpn_wH6iuZFEVXBD68i_0NixxooNsVKlJsNREiSNlY0CM4No5-tHEtRW83YLTHoPTUnDaLTjt1vPipLx0I_S_O34lhQB5BJBK0x7iH9b_VP0JREzTHw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1346494264</pqid></control><display><type>article</type><title>The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits</title><source>Springer Nature</source><creator>Pinheiro, Antônio Luiz Barbosa ; Santos, Nicole Ribeiro Silva ; Oliveira, Priscila Chagas ; Aciole, Gilberth Tadeu Santos ; Ramos, Thais Andrade ; Gonzalez, Tayná Assunção ; da Silva, Laís Nogueira ; Barbosa, Artur Felipe Santos ; Silveira, Landulfo</creator><creatorcontrib>Pinheiro, Antônio Luiz Barbosa ; Santos, Nicole Ribeiro Silva ; Oliveira, Priscila Chagas ; Aciole, Gilberth Tadeu Santos ; Ramos, Thais Andrade ; Gonzalez, Tayná Assunção ; da Silva, Laís Nogueira ; Barbosa, Artur Felipe Santos ; Silveira, Landulfo</creatorcontrib><description>The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm
2
= 16 J/cm
2
,
ϕ
= 0.5 cm
2
, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm
2
, 16 J/cm
2
= 112 J/cm
2
). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (
p
< 0.05). Pearson correlation was negative and significant (
R
2
= −
0.60
; p
<
0.001), and it was indicative that, when the Raman peaks of calcium hydroxyapatite (CHA) are increased, the level of fluorescence is reduced. It is concluded that the use of near-infrared lasertherapy associated to HA graft and GBR was effective in improving bone healing on fractured bones as a result of the increasing deposition of CHA measured by Raman spectroscopy and decrease of the organic components as shown by the fluorescence readings.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-012-1166-4</identifier><identifier>PMID: 22833288</identifier><identifier>CODEN: LMSCEZ</identifier><language>eng</language><publisher>London: Springer-Verlag</publisher><subject>Animals ; Bone Substitutes ; Bone Wires ; Bones ; Ceramics ; Dentistry ; Durapatite ; Fluorescence ; Fracture Fixation, Internal - methods ; Fractures ; Guided Tissue Regeneration - methods ; Infrared Rays - therapeutic use ; Lasers ; Lasers, Semiconductor - therapeutic use ; Light therapy ; Low-Level Light Therapy - methods ; Male ; Medicine ; Medicine & Public Health ; Optical Devices ; Optics ; Original Article ; Photonics ; Quantum Optics ; Rabbits ; Spectrum Analysis, Raman ; Tibial Fractures - radiotherapy ; Tibial Fractures - surgery</subject><ispartof>Lasers in medical science, 2013-05, Vol.28 (3), p.815-822</ispartof><rights>Springer-Verlag London Ltd 2012</rights><rights>Springer-Verlag London 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-3c45dc5a40f4a7c820e2d9bec2fe4f2d28955f3cf9e346176f4791b212368c123</citedby><cites>FETCH-LOGICAL-c448t-3c45dc5a40f4a7c820e2d9bec2fe4f2d28955f3cf9e346176f4791b212368c123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22833288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pinheiro, Antônio Luiz Barbosa</creatorcontrib><creatorcontrib>Santos, Nicole Ribeiro Silva</creatorcontrib><creatorcontrib>Oliveira, Priscila Chagas</creatorcontrib><creatorcontrib>Aciole, Gilberth Tadeu Santos</creatorcontrib><creatorcontrib>Ramos, Thais Andrade</creatorcontrib><creatorcontrib>Gonzalez, Tayná Assunção</creatorcontrib><creatorcontrib>da Silva, Laís Nogueira</creatorcontrib><creatorcontrib>Barbosa, Artur Felipe Santos</creatorcontrib><creatorcontrib>Silveira, Landulfo</creatorcontrib><title>The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm
2
= 16 J/cm
2
,
ϕ
= 0.5 cm
2
, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm
2
, 16 J/cm
2
= 112 J/cm
2
). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (
p
< 0.05). Pearson correlation was negative and significant (
R
2
= −
0.60
; p
<
0.001), and it was indicative that, when the Raman peaks of calcium hydroxyapatite (CHA) are increased, the level of fluorescence is reduced. It is concluded that the use of near-infrared lasertherapy associated to HA graft and GBR was effective in improving bone healing on fractured bones as a result of the increasing deposition of CHA measured by Raman spectroscopy and decrease of the organic components as shown by the fluorescence readings.</description><subject>Animals</subject><subject>Bone Substitutes</subject><subject>Bone Wires</subject><subject>Bones</subject><subject>Ceramics</subject><subject>Dentistry</subject><subject>Durapatite</subject><subject>Fluorescence</subject><subject>Fracture Fixation, Internal - methods</subject><subject>Fractures</subject><subject>Guided Tissue Regeneration - methods</subject><subject>Infrared Rays - therapeutic use</subject><subject>Lasers</subject><subject>Lasers, Semiconductor - therapeutic use</subject><subject>Light therapy</subject><subject>Low-Level Light Therapy - methods</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Quantum Optics</subject><subject>Rabbits</subject><subject>Spectrum Analysis, Raman</subject><subject>Tibial Fractures - radiotherapy</subject><subject>Tibial Fractures - surgery</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNktuK1TAUhosoznb0AbyRgDfeVHPqyTsZPAwMCMMI3pU0Xdk7Q9vUrHSkT-0ruDp7KyIIQkla1rf-fyX9s-y54K8F59UbFFxwlXMhcyHKMtcPsp3QqshLrr8-zHZclnVeN1KcZU8QbzkXVSnU4-xMylopWde77MfNARg4562xKwuOJfpeELbXy2s2GITI5kNIgQrRzCsziMF6k6BnKbDOzweD3jJL1ZH2fTQuMTP1bL_4nqAuTMAi7GEiIvkwMXpwiXuyHJiLxqYlArIU4V70u08HWiKNgAkCrhM5o8e3zDAbxtlsKndwGs0NS6BuC5OFe9drMxrSn8GmSPqYln7dHKPpOp_wafbImQHh2Wk_z758eH9z8Sm_-vzx8uLdVW61rlOurC56WxjNnTaVrSUH2TcdWOlAO9nLuikKp6xrQOmSrtXpqhGdFFKVtaX1PHt11J1j-LYApnb0NOUwmAnCgq1QhZSNpn_wH6iuZFEVXBD68i_0NixxooNsVKlJsNREiSNlY0CM4No5-tHEtRW83YLTHoPTUnDaLTjt1vPipLx0I_S_O34lhQB5BJBK0x7iH9b_VP0JREzTHw</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Pinheiro, Antônio Luiz Barbosa</creator><creator>Santos, Nicole Ribeiro Silva</creator><creator>Oliveira, Priscila Chagas</creator><creator>Aciole, Gilberth Tadeu Santos</creator><creator>Ramos, Thais Andrade</creator><creator>Gonzalez, Tayná Assunção</creator><creator>da Silva, Laís Nogueira</creator><creator>Barbosa, Artur Felipe Santos</creator><creator>Silveira, Landulfo</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20130501</creationdate><title>The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits</title><author>Pinheiro, Antônio Luiz Barbosa ; Santos, Nicole Ribeiro Silva ; Oliveira, Priscila Chagas ; Aciole, Gilberth Tadeu Santos ; Ramos, Thais Andrade ; Gonzalez, Tayná Assunção ; da Silva, Laís Nogueira ; Barbosa, Artur Felipe Santos ; Silveira, Landulfo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-3c45dc5a40f4a7c820e2d9bec2fe4f2d28955f3cf9e346176f4791b212368c123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Bone Substitutes</topic><topic>Bone Wires</topic><topic>Bones</topic><topic>Ceramics</topic><topic>Dentistry</topic><topic>Durapatite</topic><topic>Fluorescence</topic><topic>Fracture Fixation, Internal - methods</topic><topic>Fractures</topic><topic>Guided Tissue Regeneration - methods</topic><topic>Infrared Rays - therapeutic use</topic><topic>Lasers</topic><topic>Lasers, Semiconductor - therapeutic use</topic><topic>Light therapy</topic><topic>Low-Level Light Therapy - methods</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Quantum Optics</topic><topic>Rabbits</topic><topic>Spectrum Analysis, Raman</topic><topic>Tibial Fractures - radiotherapy</topic><topic>Tibial Fractures - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinheiro, Antônio Luiz Barbosa</creatorcontrib><creatorcontrib>Santos, Nicole Ribeiro Silva</creatorcontrib><creatorcontrib>Oliveira, Priscila Chagas</creatorcontrib><creatorcontrib>Aciole, Gilberth Tadeu Santos</creatorcontrib><creatorcontrib>Ramos, Thais Andrade</creatorcontrib><creatorcontrib>Gonzalez, Tayná Assunção</creatorcontrib><creatorcontrib>da Silva, Laís Nogueira</creatorcontrib><creatorcontrib>Barbosa, Artur Felipe Santos</creatorcontrib><creatorcontrib>Silveira, Landulfo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Database (1962 - 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Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinheiro, Antônio Luiz Barbosa</au><au>Santos, Nicole Ribeiro Silva</au><au>Oliveira, Priscila Chagas</au><au>Aciole, Gilberth Tadeu Santos</au><au>Ramos, Thais Andrade</au><au>Gonzalez, Tayná Assunção</au><au>da Silva, Laís Nogueira</au><au>Barbosa, Artur Felipe Santos</au><au>Silveira, Landulfo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>28</volume><issue>3</issue><spage>815</spage><epage>822</epage><pages>815-822</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><coden>LMSCEZ</coden><abstract>The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm
2
= 16 J/cm
2
,
ϕ
= 0.5 cm
2
, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm
2
, 16 J/cm
2
= 112 J/cm
2
). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (
p
< 0.05). Pearson correlation was negative and significant (
R
2
= −
0.60
; p
<
0.001), and it was indicative that, when the Raman peaks of calcium hydroxyapatite (CHA) are increased, the level of fluorescence is reduced. It is concluded that the use of near-infrared lasertherapy associated to HA graft and GBR was effective in improving bone healing on fractured bones as a result of the increasing deposition of CHA measured by Raman spectroscopy and decrease of the organic components as shown by the fluorescence readings.</abstract><cop>London</cop><pub>Springer-Verlag</pub><pmid>22833288</pmid><doi>10.1007/s10103-012-1166-4</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Lasers in medical science, 2013-05, Vol.28 (3), p.815-822 |
| issn | 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1352294288 |
| source | Springer Nature |
| subjects | Animals Bone Substitutes Bone Wires Bones Ceramics Dentistry Durapatite Fluorescence Fracture Fixation, Internal - methods Fractures Guided Tissue Regeneration - methods Infrared Rays - therapeutic use Lasers Lasers, Semiconductor - therapeutic use Light therapy Low-Level Light Therapy - methods Male Medicine Medicine & Public Health Optical Devices Optics Original Article Photonics Quantum Optics Rabbits Spectrum Analysis, Raman Tibial Fractures - radiotherapy Tibial Fractures - surgery |
| title | The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits |
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