Erratum to: Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit

A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular genetic methods was performed on six strains of an unknown Gram-positive, nonspore-forming, facultative anaerobic coccus-shaped bacterium isolated from a swine-manure storage pit. On the basis of 16S rRNA, RN...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2013-06, Vol.103 (6), p.1409-1418
Main Authors: Cotta, Michael A., Whitehead, Terence R., Falsen, Enevold, Moore, Edward, Lawson, Paul A.
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques
Base Composition
Base Sequence
Biomedical and Life Sciences
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Enterococcus - classification
Enterococcus - genetics
Enterococcus - isolation & purification
Enterococcus - physiology
Erratum
Fatty Acids - analysis
Feces - microbiology
Gram-positive bacteria
Hogs
Life Sciences
Manure - microbiology
Manures
Medical Microbiology
Microbiology
Molecular Sequence Data
Phylogenetics
Phylogeny
Plant Sciences
RNA polymerase
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Soil Science & Conservation
Swine
Taxonomy
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Summary:A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular genetic methods was performed on six strains of an unknown Gram-positive, nonspore-forming, facultative anaerobic coccus-shaped bacterium isolated from a swine-manure storage pit. On the basis of 16S rRNA, RNA polymerase-subunit ( rpo A), and the 60-kilodalton chaperonin (cpn60) gene sequence analyses, it was shown that all the isolates were enterococci but formed two separate lines of descent. Pairwise 16S rRNA sequence comparisons demonstrated that the two novel organisms were most closely related to each other (97.9 %) and to Enterococcus aquimarinus (97.8 %). Both organisms contained major amounts of C 16:0 , C 16:1 ω7c, and C 18:1 ω7c/12t/9t as the major cellular fatty acids. Based on biochemical, chemotaxonomic, and phylogenetic evidence, the names Enterococcus lemanii sp. nov. (type strain PC32 T  = CCUG 61260 T  = NRRL B-59661 T ) and Enterococcus eurekensis sp. nov. (type strain PC4B T  = CCUG 61259 T  = NRRL B-59662 T ) are proposed for the hitherto undescribed species.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-013-9908-2