Evolutionary conservation of the oocyte transcriptome among vertebrates and its implications for understanding human reproductive function

Cross-phylum and cross-species comparative transcriptomic analyses provide an evolutionary perspective on how specific tissues use genomic information. A significant mRNA subset present in the oocytes of most vertebrates is stabilized or stored for post-LH surge use. Since transcription is arrested...

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Bibliographic Details
Published in:Molecular human reproduction 2013-06, Vol.19 (6), p.369-379
Main Authors: Sylvestre, Eve-Lyne, Robert, Claude, Pennetier, Sophie, Labrecque, Rémi, Gilbert, Isabelle, Dufort, Isabelle, Léveillé, Marie-Claude, Sirard, Marc-André
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological Evolution
Cattle
Conserved Sequence
Embryo, Mammalian
Embryo, Nonmammalian
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Humans
Mice
Molecular Sequence Data
Oocytes - cytology
Oocytes - metabolism
Pregnancy
Sequence Homology, Amino Acid
Transcriptome
Xenopus laevis - embryology
Xenopus laevis - genetics
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Summary:Cross-phylum and cross-species comparative transcriptomic analyses provide an evolutionary perspective on how specific tissues use genomic information. A significant mRNA subset present in the oocytes of most vertebrates is stabilized or stored for post-LH surge use. Since transcription is arrested in the oocyte before ovulation, this RNA is important for completing maturation and sustaining embryo development until zygotic genome activation. We compared the human oocyte transcriptome with an oocyte-enriched subset of mouse, bovine and frog (Xenopus laevis) genes in order to evaluate similarities between species. Graded temperature stringency hybridization on a multi-species oocyte cDNA array was used to measure the similarity of preferentially expressed sequences to the human oocyte library. Identity analysis of 679 human orthologs compared with each identified official gene symbol found in the subtractive (somatic-oocyte) libraries comprising our array revealed that bovine/human similarity was greater than mouse/human or frog/human similarity. However, based on protein sequence, mouse/human similarity was greater than bovine/human similarity. Among the genes over-expressed in oocytes relative to somatic tissue in Xenopus, Mus and Bos, a high level of conservation was found relative to humans, especially for genes involved in early embryonic development.
ISSN:1360-9947
1460-2407
DOI:10.1093/molehr/gat006