Rhizobium pisi sv. trifolii K3.22 harboring nod genes of the Rhizobium leguminosarum sv. trifolii cluster

The taxonomic status of the Rhizobium sp. K3.22 clover nodule isolate was studied by multilocus sequence analysis (MLSA) of 16S rRNA and six housekeeping chromosomal genes, as well as by a subsequent phylogenic analysis. The results revealed full congruence with the Rhizobium pisi DSM 30132T core ge...

Full description

Saved in:
Bibliographic Details
Published in:Systematic and applied microbiology 2013-06, Vol.36 (4), p.252-258
Main Authors: Marek-Kozaczuk, Monika, Leszcz, Agnieszka, Wielbo, Jerzy, Wdowiak-Wróbel, Sylwia, Skorupska, Anna
Format: Article
Language:English
Subjects:
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Genes, Bacterial
Host range
Host Specificity
Medicago - microbiology
Molecular Sequence Data
Molecular Typing
Nod genes
Phylogeny
Plant Root Nodulation
Plasmids
Rhizobium leguminosarum - classification
Rhizobium leguminosarum - genetics
Rhizobium leguminosarum - isolation & purification
Rhizobium leguminosarum - physiology
Rhizobium pisi
RNA, Ribosomal, 16S - genetics
Root Nodules, Plant - microbiology
Sequence Analysis, DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The taxonomic status of the Rhizobium sp. K3.22 clover nodule isolate was studied by multilocus sequence analysis (MLSA) of 16S rRNA and six housekeeping chromosomal genes, as well as by a subsequent phylogenic analysis. The results revealed full congruence with the Rhizobium pisi DSM 30132T core genes, thus supporting the same taxonomic position for both strains. However, the K3.22 plasmid symbiosis nod genes demonstrated high sequence similarity to Rhizobium leguminosarum sv. trifolii, whereas the R. pisi DSM 30132Tnod genes were most similar to R. leguminosarum sv. viciae. The strains differed in the host range nodulation specificity, since strain K3.22 effectively nodulated red and white clover but not vetch, in contrast to R. pisi DSM 30132T, which effectively nodulated vetch but was not able to nodulate clover. Both strains had the ability to form nodules on pea and bean but they differed in bean cultivar specificity. The R. pisi K3.22 and DSM 30132T strains might provide evidence for the transfer of R. leguminosarum sv. trifolii and sv. viciae symbiotic plasmids occurring in natural soil populations.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2013.01.005