Composition and characteristics of basic proteins from peanut (Arachis hypogaea L.) seed

Basic proteins from peanut (Arachis hypogaea L.) seed were isolated from total protein extract by using carboxymethylcellulose (CMC) at pH 8.2. The basic proteins constituted about 1% of the total seed protein. Gel filtration of basic proteins on Sephadex G-200 showed the presence of two protein pea...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1982-01, Vol.30 (6), p.1176-1179
Main Authors: Basha, Sheikh M, Pancholy, Sunil K
Format: Article
Language:English
Subjects:
Arachis hypogaea
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Basic proteins from peanut (Arachis hypogaea L.) seed were isolated from total protein extract by using carboxymethylcellulose (CMC) at pH 8.2. The basic proteins constituted about 1% of the total seed protein. Gel filtration of basic proteins on Sephadex G-200 showed the presence of two protein peaks. Peak I had an apparent molecular weight of 70 000 plus or minus 5000 and peak II eluted in the salt volume of the column. Ion-exchange chromatography of the basic proteins on a CMC column gave one major and five minor protein peaks. The basic protein fraction was rich in lysine (8.5%), glycine (27.9%), and methionine (1%) but was low in aspartic acid (5.3%) and glutamic acid (5.6%) compared to the total seed protein. The basic proteins were found to be glycoproteins and contained both the neutral (3.5%, glucose and mannose) and amino sugars (0.2%, glucosamine).
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00114a043