Transgenic manipulation of plant embryo sacs tracked through cell-type-specific fluorescent markers: cell labeling, cell ablation, and adventitious embryos

Expression datasets relating to the Arabidopsis female gametophyte have enabled the creation of a tool set which allows simultaneous visual tracking of each specific cell type (egg, synergids, central cell, and antipodals). This cell-specific, fluorescent labeling tool-set functions from gametophyte...

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Bibliographic Details
Published in:Plant reproduction 2013-06, Vol.26 (2), p.125-137
Main Authors: Lawit, Shai J, Chamberlin, Mark A, Agee, April, Caswell, Eric S, Albertsen, Marc C
Format: Article
Language:English
Subjects:
Agriculture
agronomic traits
apomixis
Apomixis - genetics
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis - ultrastructure
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biomedical and Life Sciences
Cell Biology
data collection
eggs
Embryo
embryo (plant)
embryogenesis
Embryonic development
females
Fertilization
Fluorescence
fluorescent labeling
gametophytes
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genetic engineering
Genetic Markers
genetically modified organisms
heterosis
Life Sciences
Mutation
Organ Specificity
Original Article
ova
Ovule - genetics
Ovule - metabolism
Ovule - ultrastructure
ovules
Phenotype
Plant Sciences
Seeds - genetics
Seeds - metabolism
Seeds - ultrastructure
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Summary:Expression datasets relating to the Arabidopsis female gametophyte have enabled the creation of a tool set which allows simultaneous visual tracking of each specific cell type (egg, synergids, central cell, and antipodals). This cell-specific, fluorescent labeling tool-set functions from gametophyte cellularization through fertilization and early embryo development. Using this system, cell fates were tracked within Arabidopsis ovules following molecular manipulations, such as the ablation of the egg and/or synergids. Upon egg cell ablation, it was observed that a synergid can switch its developmental fate to become egg/embryo-like upon loss of the native egg. Also, manipulated was the fate of the somatic ovular cells, which can become egg- and embryo-like, reminiscent of adventitious embryony. These advances represent initial steps toward engineering synthetic apomixis resulting in seed derived wholly from the maternal plant. The end goal of applied apomixis research, fixing important agronomic traits such as hybrid vigor, would be a key benefit to agricultural productivity.
ISSN:2194-7953
2194-7961
DOI:10.1007/s00497-013-0215-x