Oxytocin stimulated release of PGF2α and its inhibition by a cyclooxygenase inhibitor and an oxytocin receptor antagonist from equine endometrial cultures

Uterine inflammation results in a poor uterine environment and early embryonic loss in the mare due to an inhibition of maternal recognition of pregnancy caused from increased prostaglandin F₂α (PGF₂α). Oxytocin binds to endometrial cell receptors to activate prostaglandin synthesis. An oxytocin rec...

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Bibliographic Details
Published in:Animal reproduction science 2013-06, Vol.139 (1-4), p.69-75
Main Authors: Penrod, Leah V, Allen, Ronald E, Rhoads, Michelle L, Limesand, Sean W, Arns, Mark J
Format: Article
Language:English
Subjects:
Animals
antagonists
Atosiban
Biopsy - veterinary
Cyclooxygenase
Cyclooxygenase Inhibitors
Dinoprost - metabolism
Dinoprost - secretion
embryonic mortality
Endometrium - drug effects
Endometrium - metabolism
Equine endometrium
estrus
explants
Female
Hormone Antagonists - pharmacology
Horses - metabolism
indomethacin
Indomethacin - pharmacokinetics
inflammation
Least-Squares Analysis
mares
Organ Culture Techniques
Oxytocin
Oxytocin - pharmacology
PGF2α
pregnancy
prostaglandin synthase
prostaglandins
receptors
Receptors, Oxytocin - antagonists & inhibitors
secretion
Vasotocin - analogs & derivatives
Vasotocin - pharmacology
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Summary:Uterine inflammation results in a poor uterine environment and early embryonic loss in the mare due to an inhibition of maternal recognition of pregnancy caused from increased prostaglandin F₂α (PGF₂α). Oxytocin binds to endometrial cell receptors to activate prostaglandin synthesis. An oxytocin receptor antagonist (Atosiban) and a cyclooxygenase inhibitor (indomethacin) both decrease PGF₂α production. The aim of this study was to evaluate the in vitro effects of Atosiban and indomethacin on equine uterine prostaglandin secretion. Equine endometrial explants were harvested on day two of behavioral estrus. Endometrial explant cultures were challenged with oxytocin (250nM) and PGF₂α concentrations were measured over time. Explants were also cultured with Atosiban and indomethacin for 6h to determine the influence on PGF₂α secretion. When endometrial explants were challenged with oxytocin, PGF₂α concentrations were greater (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2013.04.010