Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-09, Vol.109, p.228-235
• Main Authors: Gonçalves, Sara, Leirós, Ana, van Kooten, Theo, Dourado, Fernando, Rodrigues, Lígia R.
• Format: Article
• Language: English
• Subjects: adhesion; ambient temperature; Anti-adhesion; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Chemistry, Physical; colloids; contact angle; Cytotoxicity; Dimethylpolysiloxanes - chemistry; Dimethylpolysiloxanes - pharmacology; ethane; ethylene glycol; fibroblasts; Fibroblasts - cytology; Fourier transform infrared spectroscopy; Humans; hydrophilicity; hydroxylation; in vitro studies; Microbial Sensitivity Tests; ozone; Poly(dimethyl siloxane); polyethylene glycol; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacology; Polymerization; prostheses; Prostheses and Implants; scanning electron microscopy; Science & Technology; Skin - cytology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Streptococcus - drug effects; Streptococcus salivarius; Structure-Activity Relationship; Surface Properties; Surface-initiated atom transfer radical polymerization; vapors; X-ray photoelectron spectroscopy
• ISSN: 0927-7765; 1873-4367; 0927-7765
• DOI: 10.1016/j.colsurfb.2013.03.050

•Poly(dimethyl siloxane) (PDMS) was successfully modified with PEGMA by surface-initiated atom transfer radical polymerization (ATRP).•Modified surfaces showed a hydrophilic character with water contact angles around 60°.•Bacterial adhesion onto the modified surfaces of Streptococcus salivarius GB 24/9 and Staphylococcus aureus GB 2/1 was inhibited by 81–94%, respectively.•Modified surfaces showed no evidence of cytotoxic effects on human skin fibroblasts cultures. Poly(dimethyl siloxane) (PDMS) was surface-polymerized with poly(ethylene glycol)methacrylate (PEGMA) by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous media at room temperature. Modification of the PDMS surface followed a three-step procedure: (i) PDMS surface hydroxylation by UV/ozone exposure, immediately followed by (ii) covalent attachment of the initiator, 1-trichlorosilyl-2-(chloromethylphenyl)ethane, onto the hydroxylated PDMS, via chemical vapor deposition; finally (iii) PDMS surface-polymerization of PEGMA by ATRP. Modified PDMS was characterized by water contact angle measurement, SEM, FTIR-ATR, and XPS. Results showed that modified surfaces had a hydrophilic character, given the water contact angles around 60°; FTIR-ATR and XPS analysis confirmed the presence of polymerized PEGMA on the surface of PDMS and the adhesion of Staphylococcus aureus GB 2/1 and Streptococcus salivarius GB 24/9 onto the modified surfaces was inhibited 94% and 81%, respectively. Finally, the modified PDMS showed no evidence of cytotoxic effects in in vitro assays using human skin fibroblasts.