Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs

Highlights ► The study was performed using a DEP-based device to detect and sort 100% pure CTCs. ► Analyses were conducted on blood from 40 patients with metastatic colon cancer. ► CTCs were found in 21 patients. ► A difference in KRAS status between CTCs and primary tumor was observed in 8 cases. ►...

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Bibliographic Details
Published in:Cancer letters 2013-07, Vol.335 (1), p.225-231
Main Authors: Fabbri, Francesco, Carloni, Silvia, Zoli, Wainer, Ulivi, Paola, Gallerani, Giulia, Fici, Pietro, Chiadini, Elisa, Passardi, Alessandro, Frassineti, Giovanni L, Ragazzini, Angela, Amadori, Dino
Format: Article
Language:English
Subjects:
Aged
blood
Case-Control Studies
Cell Line, Tumor
Cell Separation - methods
Centrifugation, Density Gradient
Colonic Neoplasms - pathology
Colorectal cancer
colorectal neoplasms
CTCs
Dielectrophoresis
Electrophoresis
Female
genes
Hematology, Oncology and Palliative Medicine
Heterogeneity
Humans
instrumentation
Keratins - metabolism
KRAS
Male
metastasis
Middle Aged
mutation
Mutation, Missense
Neoplastic Cells, Circulating - metabolism
patients
Prospective Studies
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins p21(ras)
ras Proteins - genetics
Sensitivity and Specificity
Sequence Analysis, DNA
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Summary:Highlights ► The study was performed using a DEP-based device to detect and sort 100% pure CTCs. ► Analyses were conducted on blood from 40 patients with metastatic colon cancer. ► CTCs were found in 21 patients. ► A difference in KRAS status between CTCs and primary tumor was observed in 8 cases. ► Our DEP-based assay invites reflection on the value of CTC characterization.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2013.02.015