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Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs

Highlights ► The study was performed using a DEP-based device to detect and sort 100% pure CTCs. ► Analyses were conducted on blood from 40 patients with metastatic colon cancer. ► CTCs were found in 21 patients. ► A difference in KRAS status between CTCs and primary tumor was observed in 8 cases. ►...

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Published in:	Cancer letters 2013-07, Vol.335 (1), p.225-231
Main Authors:	Fabbri, Francesco, Carloni, Silvia, Zoli, Wainer, Ulivi, Paola, Gallerani, Giulia, Fici, Pietro, Chiadini, Elisa, Passardi, Alessandro, Frassineti, Giovanni L, Ragazzini, Angela, Amadori, Dino
Format:	Article
Language:	English
Subjects:	Aged blood Case-Control Studies Cell Line, Tumor Cell Separation - methods Centrifugation, Density Gradient Colonic Neoplasms - pathology Colorectal cancer colorectal neoplasms CTCs Dielectrophoresis Electrophoresis Female genes Hematology, Oncology and Palliative Medicine Heterogeneity Humans instrumentation Keratins - metabolism KRAS Male metastasis Middle Aged mutation Mutation, Missense Neoplastic Cells, Circulating - metabolism patients Prospective Studies Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins p21(ras) ras Proteins - genetics Sensitivity and Specificity Sequence Analysis, DNA
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creator	Fabbri, Francesco Carloni, Silvia Zoli, Wainer Ulivi, Paola Gallerani, Giulia Fici, Pietro Chiadini, Elisa Passardi, Alessandro Frassineti, Giovanni L Ragazzini, Angela Amadori, Dino
description	Highlights ► The study was performed using a DEP-based device to detect and sort 100% pure CTCs. ► Analyses were conducted on blood from 40 patients with metastatic colon cancer. ► CTCs were found in 21 patients. ► A difference in KRAS status between CTCs and primary tumor was observed in 8 cases. ► Our DEP-based assay invites reflection on the value of CTC characterization.
doi_str_mv	10.1016/j.canlet.2013.02.015
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subjects	Aged blood Case-Control Studies Cell Line, Tumor Cell Separation - methods Centrifugation, Density Gradient Colonic Neoplasms - pathology Colorectal cancer colorectal neoplasms CTCs Dielectrophoresis Electrophoresis Female genes Hematology, Oncology and Palliative Medicine Heterogeneity Humans instrumentation Keratins - metabolism KRAS Male metastasis Middle Aged mutation Mutation, Missense Neoplastic Cells, Circulating - metabolism patients Prospective Studies Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins p21(ras) ras Proteins - genetics Sensitivity and Specificity Sequence Analysis, DNA
title	Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs
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