Development of a biosensor based on immobilization of acetylcholinesterase on NiO nanoparticles–carboxylic graphene–nafion modified electrode for detection of pesticides

• Published in: Talanta (Oxford) 2013-09, Vol.113, p.135-141
• Main Authors: Yang, Long, Wang, Guangcan, Liu, Yongjun, Wang, Min
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase; Acetylcholinesterase - chemistry; adhesion; Amperometric biosensor; biocompatibility; Biosensing Techniques; biosensors; carbofuran; Carbofuran - analysis; Carboxylic graphene; catalytic activity; Chitosan - chemistry; chlorpyrifos; Chlorpyrifos - analysis; Cholinesterase Inhibitors - analysis; detection limit; Electrodes; enzyme inhibitors; enzyme kinetics; Enzymes, Immobilized - chemistry; Fluorocarbon Polymers - chemistry; glassy carbon electrode; graphene; Graphite - chemistry; hydrolysis; hydrophilicity; Membranes, Artificial; Metal Nanoparticles - chemistry; Methyl Parathion - analysis; nanocomposites; Nanocomposites - chemistry; nanoparticles; Nickel - chemistry; NiO nanoparticles; parathion-methyl; Pesticides; Pesticides - analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2013.03.025

A sensitive amperometric acetylcholinesterase (AChE) biosensor based on NiO nanoparticles (NiO NPs), carboxylic graphene (CGR) and nafion (NF) modified glassy carbon electrode (GCE) has been developed. NiO NPs-CGR-NF nanocomposites with excellent conductivity, catalysis and biocompatibility offered an extremely hydrophilic surface for AChE adhesion. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl with an apparent Michaelis–Menten constant value of 135μM. Under optimum conditions, the biosensor detected methyl parathion and chlorpyrifos in the linear range from 1.0×10−13 to 1×10−10M and from 1.0×10−10 to 1×10−8M with the detection limits 5×10−14M. The biosensor detected carbofuran in the linear range from 1.0×10−12 to 1×10−10M and from 1.0×10−10 to 1×10−8M with the detection limit of 5×10−13M. The developed biosensor exhibited good sensitivity, stability and reproducibility, thus providing a promising tool for analysis of enzyme inhibitors. [Display omitted] •The nanocomposite of NiO nanoparticles and carboxylic graphene is synthesized.•The biosensor based on NiO nanoparticles–carboxylic graphene is developed.•Chitosan is used to immobilize AChE and nafion used as a protective membrane.•The biosensor exhibits high sensitivity and acceptable stability.