Investigation of DNA changes in wheat (Triticum aestivum L.) induced by cadmium using random amplified polymorphic DNA (RAPD) analysis

In recent years, many plants have been used as bioindicators or living indices and several experiments have been conducted to evaluate the genotoxic effects of environmental pollution on plant species. Plants such as Arabidopsis and barley have been used as biological indicators by several researche...

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Bibliographic Details
Published in:African journal of biotechnology 2013-04, Vol.12 (16), p.1921-1929
Main Authors: Akbar, Azimi, Farajollah, Shahriari, Amir, Fotovat, Reza, Kazemi Qale, Khodaverd, Hajizade Agje
Format: Article
Language:English
Subjects:
Arabidopsis
Hordeum vulgare
Triticum aestivum
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Summary:In recent years, many plants have been used as bioindicators or living indices and several experiments have been conducted to evaluate the genotoxic effects of environmental pollution on plant species. Plants such as Arabidopsis and barley have been used as biological indicators by several researchers and have been able to identify DNA changes using molecular markers. This study was conducted on wheat as an important cereal that provides human daily food and has an important role in human health. The purpose of study was to evaluate the effect of different concentrations of cadmium on amounts of the soluble proteins, investigation of growth and possible changes to the structure of DNA using random amplified polymorphic DNA (RAPD) marker. In the present study, wheat seedlings were used for detection of genotoxic effects of cadmium contamination in the range of 0 to 120 mgl super(1). The limiting effects of cadmium on length of root and plant height and total soluble proteins in root were quite evident by increase of cadmium concentration. Change in DNA was observed using RAPD marker as there was change in number of bands, present or absent of bands at the range of above 5 mgl super(1) cadmium concentration. In the previous studies conducted by several researchers, variation in DNA bands was reported at levels above 30 mgl super(1), whereas in the present study, band variation was observed at the concentration of 5 mgl super(1). This study also showed that DNA stability is highly affected by cadmium pollution at >5 mgl super(1) which was identified by RAPD markers.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB12.2836