Zymogram profiling of superoxide dismutase and catalase activities allows Saccharomyces and non-Saccharomyces species differentiation and correlates to their fermentation performance

Aerobic organisms have devised several enzymatic and non-enzymatic antioxidant defenses to deal with reactive oxygen species (ROS) produced by cellular metabolism. To combat such stress, cells induce ROS scavenging enzymes such as catalase, peroxidase, superoxide dismutase (SOD) and glutathione redu...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2013-05, Vol.97 (10), p.4563-4576
Main Authors: Gamero-Sandemetrio, Esther, Gómez-Pastor, Rocío, Matallana, Emilia
Format: Article
Language:English
Subjects:
Analysis
Antioxidants
Applied Microbial and Cell Physiology
Biomass
Biomedical and Life Sciences
Biotechnology
Catalase - metabolism
Cell Differentiation
Enzymatic activity
Enzymes
Experiments
Fermentation
Laboratories
Life Sciences
Metabolites
Microbial Genetics and Genomics
Microbiology
Physiological aspects
Polyacrylamide
Saccharomyces
Saccharomyces - cytology
Saccharomyces - enzymology
Saccharomyces - metabolism
Stains & staining
Studies
Superoxide
Superoxide Dismutase - metabolism
Syrups & sweeteners
Yeast
Yeasts
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Summary:Aerobic organisms have devised several enzymatic and non-enzymatic antioxidant defenses to deal with reactive oxygen species (ROS) produced by cellular metabolism. To combat such stress, cells induce ROS scavenging enzymes such as catalase, peroxidase, superoxide dismutase (SOD) and glutathione reductase. In the present research, we have used a double staining technique of SOD and catalase enzymes in the same polyacrylamide gel to analyze the different antioxidant enzymatic activities and protein isoforms present in Saccharomyces and non- Saccharomyces yeast species. Moreover, we used a technique to differentially detect Sod1p and Sod2p on gel by immersion in NaCN, which specifically inhibits the Sod1p isoform. We observed unique SOD and catalase zymogram profiles for all the analyzed yeasts and we propose this technique as a new approach for Saccharomyces and non- Saccharomyces yeast strains differentiation. In addition, we observed functional correlations between SOD and catalase enzyme activities, accumulation of essential metabolites, such as glutathione and trehalose, and the fermentative performance of different yeasts strains with industrial relevance.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-012-4672-1