Synapsin-induced proliferation of T-cell lines against myelin basic protein obtained from rats with experimental autoimmune encephalomyelitis

• Published in: Autoimmunity (Chur, Switzerland) Switzerland), 2009-11, Vol.42 (8), p.661-666
• Main Authors: Degano, Alicia L., Roth, German A.
• Format: Article
• Language: English
• Subjects: Animals; autoimmunity; Cell Line; Cell Proliferation; Cross Reactions - immunology; cytokines; Encephalomyelitis, Autoimmune, Experimental - immunology; experimental autoimmune encephalomyelitis; Female; Guinea Pigs; Interferon-gamma - metabolism; Interleukin-10 - metabolism; Interleukin-2 - metabolism; Interleukin-4 - metabolism; Lymph Nodes - cytology; Lymph Nodes - immunology; Lymphocyte Activation - immunology; myelin basic protein; Myelin Basic Protein - immunology; Peptide Fragments - genetics; Peptide Fragments - immunology; Rats; Rats, Inbred Lew; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Spleen - cytology; Spleen - immunology; synapsin; Synapsins - genetics; Synapsins - immunology; synaptosomal antigens; T-Lymphocytes - cytology; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Tuberculin - immunology; Vaccination
• ISSN: 0891-6934; 1607-842X
• DOI: 10.3109/08916930903120958

We have previously described that antibodies and T cells against myelin basic protein (MBP) rose under conditions to induce acute experimental autoimmune encephalomyelitis (EAE) bind other proteins present in the synaptosomal fraction, some of them identified as synapsin I. The aim of this study was to evaluate whether anti-MBP T-cell lines can be also activated by synapsin. The analysis of rat anti-MBP T-cell lines cultured with each antigen showed that these cells responded also to purified rat synapsin and to the amino terminal portion of this protein. This recognition originated a proliferative response with a concomitant pattern of cytokine secretion similar to that induced by MBP itself implicating that this recognition would be mediated by the T-cell receptor. On the other hand, anti-synapsin T-cell lines were not capable of responding to MBP stimulation. Therefore, the immunological cross-reactivity between both proteins occurs only in one direction and these cross-reactive cells would be elicited only in animals sensitized with MBP. A possible implication of immunological agents against MBP cross-reactive with extra-myelin proteins in the process of EAE is considered.