Protein destabilizing agents induce polyreactivity and enhanced immunomodulatory activity in IVIg preparations

Normal pooled human IVIg are produced using various blood protein fractionation technologies and as a result they may well differ in their biological properties. We have demonstrated that exposure of IVIg, for a period as short as 15 min, to protein-destabilizing agents like acidic pH, ROS or pro-ox...

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Bibliographic Details
Published in:Autoimmunity (Chur, Switzerland) Switzerland), 2009-01, Vol.42 (4), p.365-367
Main Authors: Djoumerska-Alexieva, Iglika K., Dimitrov, Jordan D., Nacheva, Julia, Kaveri, Srini V., Vassilev, Tchavdar L.
Format: Article
Language:English
Subjects:
Animals
Antibody Specificity - drug effects
Antibody Specificity - immunology
Chromatography, High Pressure Liquid
Disease Models, Animal
Humans
Immunoglobulins, Intravenous - chemistry
Immunoglobulins, Intravenous - drug effects
Immunoglobulins, Intravenous - immunology
Iron - pharmacology
Mice
Mice, Inbred ICR
Oxidation-Reduction
Reactive Oxygen Species
Shock, Septic - drug therapy
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Summary:Normal pooled human IVIg are produced using various blood protein fractionation technologies and as a result they may well differ in their biological properties. We have demonstrated that exposure of IVIg, for a period as short as 15 min, to protein-destabilizing agents like acidic pH, ROS or pro-oxidative ferrous ions dramatically increases the panel of recognized Ag including pro-inflammatory cytokines. We now show that exposure of IVIg to ferrous ions modifies some IgG molecules without denaturating them and enhances the protective activity of the preparation in experimental septic shock.
ISSN:0891-6934
1607-842X
DOI:10.1080/08916930902832181