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Protein destabilizing agents induce polyreactivity and enhanced immunomodulatory activity in IVIg preparations

Normal pooled human IVIg are produced using various blood protein fractionation technologies and as a result they may well differ in their biological properties. We have demonstrated that exposure of IVIg, for a period as short as 15 min, to protein-destabilizing agents like acidic pH, ROS or pro-ox...

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Published in:	Autoimmunity (Chur, Switzerland) Switzerland), 2009-01, Vol.42 (4), p.365-367
Main Authors:	Djoumerska-Alexieva, Iglika K., Dimitrov, Jordan D., Nacheva, Julia, Kaveri, Srini V., Vassilev, Tchavdar L.
Format:	Article
Language:	English
Subjects:	Animals Antibody Specificity - drug effects Antibody Specificity - immunology Chromatography, High Pressure Liquid Disease Models, Animal Humans Immunoglobulins, Intravenous - chemistry Immunoglobulins, Intravenous - drug effects Immunoglobulins, Intravenous - immunology Iron - pharmacology Mice Mice, Inbred ICR Oxidation-Reduction Reactive Oxygen Species Shock, Septic - drug therapy
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cited_by	cdi_FETCH-LOGICAL-c377t-c9e362636d03d12fdd594f0c31945be1fd5fea2088f9a6acf2a891b5087dbb113
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container_end_page	367
container_issue	4
container_start_page	365
container_title	Autoimmunity (Chur, Switzerland)
container_volume	42
creator	Djoumerska-Alexieva, Iglika K. Dimitrov, Jordan D. Nacheva, Julia Kaveri, Srini V. Vassilev, Tchavdar L.
description	Normal pooled human IVIg are produced using various blood protein fractionation technologies and as a result they may well differ in their biological properties. We have demonstrated that exposure of IVIg, for a period as short as 15 min, to protein-destabilizing agents like acidic pH, ROS or pro-oxidative ferrous ions dramatically increases the panel of recognized Ag including pro-inflammatory cytokines. We now show that exposure of IVIg to ferrous ions modifies some IgG molecules without denaturating them and enhances the protective activity of the preparation in experimental septic shock.
doi_str_mv	10.1080/08916930902832181
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subjects	Animals Antibody Specificity - drug effects Antibody Specificity - immunology Chromatography, High Pressure Liquid Disease Models, Animal Humans Immunoglobulins, Intravenous - chemistry Immunoglobulins, Intravenous - drug effects Immunoglobulins, Intravenous - immunology Iron - pharmacology Mice Mice, Inbred ICR Oxidation-Reduction Reactive Oxygen Species Shock, Septic - drug therapy
title	Protein destabilizing agents induce polyreactivity and enhanced immunomodulatory activity in IVIg preparations
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