Nucleotide and phylogenetic analysis of human papillomavirus types 6 and 11 isolated from recurrent respiratory papillomatosis in Brazil

• Published in: Infection, genetics and evolution genetics and evolution, 2013-06, Vol.16, p.282-289
• Main Authors: de Matos, Renata Prandini Adum, Sichero, Laura, Mansur, Isabela Mazuco, do Bonfim, Caroline Measso, Bittar, Cíntia, Nogueira, Rodrigo Lacerda, Küpper, Daniel Salgado, Valera, Fabiana Cardoso Pereira, Nogueira, Maurício Lacerda, Villa, Luisa Lina, Calmon, Marilia Freitas, Rahal, Paula
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Brazil; Child; Child, Preschool; Cohort Studies; disease severity; Female; genes; geographical distribution; HPV variants; HPV-11; HPV-6; Human papillomavirus 11 - classification; Human papillomavirus 11 - genetics; Human papillomavirus 6 - classification; Human papillomavirus 6 - genetics; Humans; larynx; Male; Middle Aged; Mutation; nucleotide sequences; papilloma; Papillomavirus Infections - virology; patients; Phylogenetic analysis; Phylogeny; Recurrent respiratory papillomatosis; Respiratory Tract Infections - virology; uterine cervical neoplasms; Viral Proteins - chemistry; Viral Proteins - genetics; warts
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2012.12.033

► E6 variability of low risk HPVs detected in recurrent respiratory papillomatosis. ► Four different HPV-6 genomic variants and two HPV-11 genomic variants were identified. ► The sequences from Brazil did not group together. There are few studies about the distribution of natural molecular variants of low-risk HPVs. Our aim was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. We also performed a phylogenetic analysis in order to compare nucleotide sequences identified in our study with previously reported isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world to determine the phylogenetic relationships of variants detected in Brazil. The complete coding region of the E6 gene of 25 samples was cloned and sequenced: 18 isolates of HPV-6 (72%) and 7 isolates of HPV-11 (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants was identified. It was not possible to correlate specific variants with disease severity. Phylogenetic trees for both HPV types were constructed enclosing both E6 sequences detected in our study and formerly published sequences. In both phylogenetic trees, the sequences from Brazil did not group together. We could not establish a geographical association between HPV-6 or HPV-11 variants, unlike HPV-16 and HPV-18.