Association of vascular endothelial growth factor ( VEGF ) gene polymorphism and increased serum VEGF concentration with pancreatic adenocarcinoma

• Published in: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2013-05, Vol.13 (3), p.267-272
• Main Authors: Sivaprasad, Siddapuram, Govardhan, Bale, Harithakrishna, Ramanujam, Venkat Rao, Guduru, Pradeep, Rebala, Kunal, Bharadhwaj, Ramakrishna, Nalla, Anuradha, Shekaran, Reddy, Duvvuru Nageshwar
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - blood; Adenocarcinoma - genetics; Angiogenesis; Endocrinology & Metabolism; Female; Gastroenterology and Hepatology; Genes; Genotype–phenotype correlation; Haplotypes; Humans; Male; Middle Aged; Mortality; Pancreatic cancer; Pancreatic Neoplasms - blood; Pancreatic Neoplasms - genetics; Polymorphism; Polymorphism, Single Nucleotide; Single nucleotide polymorphisms (SNPs); Vascular endothelial growth factor; Vascular endothelial growth factor (VEGF); Vascular Endothelial Growth Factor A - blood; Vascular Endothelial Growth Factor A - genetics
• ISSN: 1424-3903; 1424-3911
• DOI: 10.1016/j.pan.2013.02.006

Abstract Background &aim Pancreatic cancer is related to high mortality rate. The vascular endothelial growth factor (VEGF) has a strong influence in tumor-related angiogenesis having association with the grade of angiogenesis and the prognosis of different solid tumors including pancreatic cancer. The present study was aimed to analyze the genotype and haplotype distribution of VEGF gene single nucleotide polymorphisms (SNPs), −460T/C, +405G/C, +936C/T, in patients with pancreatic adenocarcinoma from South India, and the effect of these SNPs on serum VEGF level. Methods Total 80 patients with pancreatic adenocarcinoma and 87 controls were recruited. The genotype of VEGF gene polymorphisms was determined in both patients and controls using polymerase chain reaction-restriction fragment length polymorphism method. The serum VEGF protein was estimated by standard enzyme-linked immunosorbent assay. Results The genotype, +405G/G of VEGF gene showed a significant association with the patients with pancreatic adenocarcinoma ( P  = 0.012, Odds ratio: 2.133), whereas no significant difference was found in the genotype distribution of SNPs, −460C/T and +936C/T between patient and control groups ( P  > 0.05). Serum VEGF level was found to be significantly high in patients (1315.10 pg/Ml, SD ± 230.79) when compared to controls (591.35 pg/mL, SD ± 92.48) ( P