Preparation and physicochemcial and immunological characterization of polysaccharide-outer membrane protein complexes of Neisseria meningitidis

A crude complex containing group C polysaccharide, outer membrane proteins, and lipopolysaccharide (LPS) was isolated from the cell-free culture liquid of N. meningitidis serogroup C, serotype 2a. Group C polysaccharide and LPS were removed from this complex, resulting in an outer membrane complex a...

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Bibliographic Details
Published in:Infection and immunity 1983-01, Vol.40 (1), p.369-380
Main Authors: Beuvery, E C, Miedema, F, van Delft, RW, Haverkamp, J, Leussink, AB, Te Pas, BJ, Teppema, K S, Tiesjema, R H
Format: Article
Language:English
Subjects:
Neisseria meningitidis
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Summary:A crude complex containing group C polysaccharide, outer membrane proteins, and lipopolysaccharide (LPS) was isolated from the cell-free culture liquid of N. meningitidis serogroup C, serotype 2a. Group C polysaccharide and LPS were removed from this complex, resulting in an outer membrane complex and a purified complex, respectively. Analysis by electron microscopy showed the outer membrane origin of the crude complex and the outer membrane complex, whereas such a structure was absent in the purified complex. Sodium dodecyl sulfate-polyacrylamide gel electrophorsis patterns of the three complex. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of the three complexes were identical. Pyrolysis-mass spectrometry data correlated well with those obtained by the biochemical assays and suggested a low LPS content in the purified complex and a low polysaccharide content in the outer membrane complex. The immunogenic activities of the complexes were studied in mice. The antibodies were measured by the enzyme-linked immunosorbent assay and the bactericidal antibody assay.
ISSN:0019-9567