Genetic diversity of Rhizobium strains which nodulate Leucaena leucocephala [Tropical legume]

DNA homologies at 65 degree C in 0.14 phosphate buffer, pH 6.8, were determined between 24 strains of Rhizobium capable of nodulating L. leucocephala and four Rhizobium reference strains. Twenty-one strains (88%) were placed in one of four DNA homology groups. The mean relative homology within a gro...

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Bibliographic Details
Published in:Current microbiology 1983, Vol.8 (2), p.153-158
Main Author: Jarvis, BDW
Format: Article
Language:English
Subjects:
Leucaena leucocephala
Rhizobium
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Summary:DNA homologies at 65 degree C in 0.14 phosphate buffer, pH 6.8, were determined between 24 strains of Rhizobium capable of nodulating L. leucocephala and four Rhizobium reference strains. Twenty-one strains (88%) were placed in one of four DNA homology groups. The mean relative homology within a group was 65%, while the mean relative homology between groups was 20%. Thermal melting points for reassociated DNA ( Delta T sub(m(e)) values) were also measured. The lack of DNA homology between groups indicates that several very different populations of bacteria are capable of nodulating and fixing nitrogen with leucaena.
ISSN:0343-8651
1432-0991