Chemical Cocarcinogenesis With the Use of a Subclone Derived From Balb/3T3 Cells With Catechol as Cocarcinogen

This study was performed for the detection of cocarcinogens by transformation in vitro with the use of a subclone of a Balb/3T3 cell line. Dose response, cytotoxicity, and transformation studies were done with the use of an indirect-acting carcinogen, benzo[a]pyrene (B[a]P), a direct-acting alkylati...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1982-08, Vol.69 (2), p.503-508
Main Authors: Atchison, Mahalakshmi, Chu, Ching-shan, Kakunaqa, Takeo, Van Duuren, Benjamin L.
Format: Article
Language:English
Subjects:
Animals
Benzopyrenes
Catechols - toxicity
Cell Line
Cell Transformation, Neoplastic - chemically induced
Clone Cells
Cocarcinogenesis
Colony-Forming Units Assay
Dose-Response Relationship, Drug
Mice
Mice, Inbred BALB C
Propiolactone
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Summary:This study was performed for the detection of cocarcinogens by transformation in vitro with the use of a subclone of a Balb/3T3 cell line. Dose response, cytotoxicity, and transformation studies were done with the use of an indirect-acting carcinogen, benzo[a]pyrene (B[a]P), a direct-acting alkylating carcinogen, β-propiolactone (BPL), and the mouse skin cocarcinogen catechol. The rate of transformation was notably higher in groups treated with B[a]P and catechol or BPL and catechol than in groups treated with either B[a]P or BPL. Catechol alone did not induce any transformation. All the cells isolated from the transformed foci showed characteristics of malignantly transformed cells, such as anchorage-independent growth. Thus chemical cocarcinogenesis was accomplished in vitro similar to that accomplished in in vivo studies reported earlier on mouse skin.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/69.2.503