In vitro protein synthesis directed by polyadenylated RNA from Bacillus brevis

Recently the authors have reported an improved method for isolating highly active polysomes from B. brevis (ATCC9999), which contained poly(A)RNA in a polydisperse distribution. The authors proposed that this poly(A)RNA is mRNA. They present evidence that the amount of poly(A)RNA in the exponential-...

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Bibliographic Details
Published in:FEMS microbiology letters 1983-05, Vol.18 (3), p.245-251
Main Authors: Altmann, Michael, Kaeufer, Norbert, Doehren, Hans
Format: Article
Language:English
Subjects:
Bacillus brevis
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Summary:Recently the authors have reported an improved method for isolating highly active polysomes from B. brevis (ATCC9999), which contained poly(A)RNA in a polydisperse distribution. The authors proposed that this poly(A)RNA is mRNA. They present evidence that the amount of poly(A)RNA in the exponential-phase increases towards the transient phase of growth, i.e. the deceleration phase between exponential growth and the stationary phase. Coding sequences present in the poly(A)RNA from B. brevis are examined by in vitro translation in a highly efficient cell-free system. Newly synthesized polypeptides analysed on autoradiograms indicate that poly(A)RNA is indeed mRNA and that polypeptides encoded by poly(A)RNA seem to be identical with those encoded by poly(A)-deficient RNA.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1983.tb00486.x