Determination of the spin state of iron in native and activated soybean lipoxygenase 1 by paramagnetic susceptibility

The paramagnetic susceptibility of the iron present in soybean lipoxygenase 1 was measured by proton nuclear magnetic resonance. Paramagnetic iron was found in both the native and activated forms of the enzyme. No paramagnetic shifts were seen with the apoenzyme nor when the native enzyme was inacti...

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Bibliographic Details
Published in:Biochemistry (Easton) 1983-08, Vol.22 (16), p.3837-3840
Main Authors: Cheesbrough, Thomas M, Axelrod, Bernard
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
E.S.R
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Glycine max
iron
lipoxygenase
Oxidoreductases
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Summary:The paramagnetic susceptibility of the iron present in soybean lipoxygenase 1 was measured by proton nuclear magnetic resonance. Paramagnetic iron was found in both the native and activated forms of the enzyme. No paramagnetic shifts were seen with the apoenzyme nor when the native enzyme was inactivated by incubation with excess linoleic acid under anaerobic conditions. Evaluation of the observed shifts indicates the enzyme contains high-spin Fe(III) in the activated state, as expected from its known electron paramagnetic resonance characteristics, and high-spin Fe(II) in the native state.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00285a019