Combination therapy of human adipose-derived stem cells and basic fibroblast growth factor hydrogel in muscle regeneration

Abstract Skeletal muscle regeneration after sport injury is inconsistent, and complete healing without fibrosis is very important. In this study, we determined whether the combination therapy using human adipose-derived stem cells (h-ADSCs) and basic fibroblast growth factor (bFGF) incorporated into...

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Bibliographic Details
Published in:Biomaterials 2013-08, Vol.34 (25), p.6037-6045
Main Authors: Hwang, Ji Hye, Kim, In Gul, Piao, Shuyu, Jung, Ae Ryang, Lee, Ji Young, Park, Ki Dong, Lee, Ji Youl
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Advanced Basic Science
Animals
bFGF
Blotting, Western
Cell Differentiation - drug effects
Dentistry
Female
Fibroblast Growth Factor 2 - chemistry
Fibroblast Growth Factor 2 - pharmacology
Humans
Hydrogel
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Immunohistochemistry
Mice
Mice, Inbred BALB C
Muscle Contraction - drug effects
Muscle, Skeletal - drug effects
Muscle, Skeletal - physiology
Regeneration - drug effects
Skeletal muscle
Stem cell
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Summary:Abstract Skeletal muscle regeneration after sport injury is inconsistent, and complete healing without fibrosis is very important. In this study, we determined whether the combination therapy using human adipose-derived stem cells (h-ADSCs) and basic fibroblast growth factor (bFGF) incorporated into hydrogel could enhance muscle regeneration in a muscle laceration animal model. The h-ADSCs and/or bFGF hydrogels were applied to the lacerated gastrocnemius muscle. Fast twitch muscle contraction improved significantly and fibrosis decreased significantly in combined h-ADSC and bFGF-hydrogel group compared to other experimental groups. Skeletal muscle differentiation of h-ADSCs was determined by immunohistochemistry (PKH-26/MyHC co-staining) and Western blot. Our data suggested that combination therapy of h-ADSCs and bFGF hydrogel resulted in functional recovery, revascularization and reinnervation with minimal fibrosis in lacerated muscle. A combination of h-ADSCs and bFGF hydrogel can be used as a promising therapy for skeletal muscle regeneration.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.04.049