Differentiation Between Analyte Adsorption and Homogenous Index Sensing in WGM Biodetection

We propose new parameters to characterize a whispering gallery mode spectrum, which can be regarded as reliable independent biodetection indicators for an instantaneous microsphere's spectrum. With these parameters, since there is no need for spectral comparison to an analyte-free reference, th...

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Bibliographic Details
Published in:IEEE sensors journal 2013-01, Vol.13 (1), p.229-233
Main Authors: Charlebois, M., Paquet, A., Verret, L. S., Boissinot, K., Boissinot, M., Bergeron, M. G., Allen, C. N.
Format: Article
Language:English
Subjects:
Bacteria
Biomedical optical imaging
Biosensors
detection algorithms
Fluidics
Fluorescence
IMARI
Microspheres
Monitoring
Refractive index
Reliability
Spectra
Spores
Three dimensional
Whispering gallery modes
whispering gallery modes (WGM)
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Summary:We propose new parameters to characterize a whispering gallery mode spectrum, which can be regarded as reliable independent biodetection indicators for an instantaneous microsphere's spectrum. With these parameters, since there is no need for spectral comparison to an analyte-free reference, three-dimensional stirring of fluorescent microspheres in fluidic setup is allowed. Through an analysis of fluorescent microspheres in the presence of bacterial spores, we found five insightful quantitative independent parameters for biodetection. Unveiled by this analysis, we demonstrate the possibility to discriminate between analyte presence and homogeneous variation of the solution's refractive index.
ISSN:1530-437X
1558-1748
DOI:10.1109/JSEN.2012.2211231