Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel close-tubed and accurate tool for olive oil forensic use

BACKGROUND The adulteration of high‐priced olive oil with low‐cost oils and the fraudulent labelling of oil products make the identification and traceability of vegetable oil species in the food chain very important. This paper describes a high‐resolution melting analysis‐based method using chloropl...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2013-07, Vol.93 (9), p.2281-2286
Main Authors: Ganopoulos, Ioannis, Bazakos, Christos, Madesis, Panagiotis, Kalaitzis, Panagiotis, Tsaftaris, Athanasios
Format: Article
Language:English
Subjects:
adulteration
Bar codes
Bar-HRM
Brassica napus - chemistry
Brassica napus - metabolism
Canola
Canola Oil
Chloroplasts
Descriptive labeling
DNA Barcoding, Taxonomic
DNA, Plant - analysis
DNA, Plant - metabolism
Fatty Acids, Monounsaturated - analysis
Fatty Acids, Monounsaturated - chemistry
Food Contamination
food forensics
Food Inspection - methods
Food Labeling
Forensic Toxicology - methods
Fraud
Fruit - chemistry
Fruit - metabolism
Greece
Limit of Detection
Nucleic Acid Denaturation
Olea - chemistry
Olea - metabolism
Olive Oil
Plant Oils - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
quantification
Real-Time Polymerase Chain Reaction
Ribulose-Bisphosphate Carboxylase - genetics
Ribulose-Bisphosphate Carboxylase - metabolism
Vegetable oils
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Summary:BACKGROUND The adulteration of high‐priced olive oil with low‐cost oils and the fraudulent labelling of oil products make the identification and traceability of vegetable oil species in the food chain very important. This paper describes a high‐resolution melting analysis‐based method using chloroplast barcoding regions as target (Bar‐HRM) to obtain barcoding information for the major vegetable oil species and to quantitatively identify the botanical origin of plant oils. The detection of adulteration of olive oil with canola oil was used as a case study. RESULTS The proposed method was capable of distinguishing among different vegetable oil species and detecting a level of 1% (w/w) of canola oil in olive oil. CONCLUSION Bar‐HRM analysis is a more accurate, faster and less costly alternative method to authenticate vegetable oils, including olive oil, and to detect mixtures of oils. © 2013 Society of Chemical Industry
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.6040