Glycoproteomic analysis of tissues from patients with colon cancer using lectin microarrays and nanoLC-MS/MS

The current study evaluated the glycoproteomic profile of tissues from colon cancer patients. The lectin microarray was first performed to compare the glycoprotein profiles between colon cancer and matched normal tissues. Level of N-acetylglucosamine (GlcNAc) that Solanum tuberosum lectin (STL) boun...

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Bibliographic Details
Published in:Molecular bioSystems 2013-01, Vol.9 (7), p.1877-1887
Main Authors: Li, Yangguang, Wen, Ti, Zhu, Minzhi, Li, Lixin, Wei, Jun, Wu, Xiaoli, Guo, Mingzhou, Liu, Shuangping, Zhao, Huiyuan, Xia, Siyuan, Huang, Weili, Wang, Puyue, Wu, Zhenzhou, Zhao, Liqing, Shui, Wenqing, Li, Zheng, Yin, Zhinan
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Colon - metabolism
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Female
Glycoproteins - analysis
Glycoproteins - chemistry
Humans
Lectins - chemistry
Lectins - metabolism
Male
Middle Aged
Neoplasm Staging
Protein Array Analysis
Proteome - analysis
Proteome - chemistry
Proteomics - methods
Reproducibility of Results
Tandem Mass Spectrometry
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Summary:The current study evaluated the glycoproteomic profile of tissues from colon cancer patients. The lectin microarray was first performed to compare the glycoprotein profiles between colon cancer and matched normal tissues. Level of N-acetylglucosamine (GlcNAc) that Solanum tuberosum lectin (STL) bound was found to be elevated in colon cancer, which was verified through lectin histochemistry. The subsequent glycoproteomic analysis based on STL enrichment of glycoproteins followed by label-free quantitative nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis identified 72 proteins in high confidence. Among these proteins, 17 were exclusively detected in cancer tissues, and 14 were significantly upregulated in tumor tissues. Annexin A1 and HSP90β were chosen for further investigation by immunoprecipitation coupled with lectin blots, western blots and tissue microarrays. Both Annexin A1 and HSP90β were GlcNAcylated, and their protein expressions were elevated in colon cancer, compared to normal tissues. Moreover, specific changes of GlcNAc abundances in Annexin A1 and HSP90β suggested that tumor-specific glycan patterns could serve as candidate biomarkers of colon cancer for distinguishing cancer patients from healthy individuals.
ISSN:1742-206X
1742-2051
DOI:10.1039/c3mb00013c