Methylglyoxal-induced modifications of significant honeybee proteinous components in manuka honey: Possible therapeutic implications

Methylglyoxal (MGO) is a major antibacterial component of manuka honey. Another antibacterial component found in Revamil honey, peptide defensin1, was not identified in manuka honey. The primary aim of the study was to evaluate the content of defensin1 in honeys of different botanical origins and to...

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Bibliographic Details
Published in:Fitoterapia 2012-06, Vol.83 (4), p.671-677
Main Authors: Majtan, Juraj, Klaudiny, Jaroslav, Bohova, Jana, Kohutova, Lenka, Dzurova, Maria, Sediva, Maria, Bartosova, Maria, Majtan, Viktor
Format: Article
Language:English
Subjects:
alpha-glucosidase
antibacterial properties
Apis mellifera
Apoidea
Biological and medical sciences
Enzyme
General pharmacology
glucose oxidase
Honey
honey bees
Honeybee
hydrogen peroxide
Medical sciences
Methylglyoxal
molecular weight
peptides
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Protein modification
proteins
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Summary:Methylglyoxal (MGO) is a major antibacterial component of manuka honey. Another antibacterial component found in Revamil honey, peptide defensin1, was not identified in manuka honey. The primary aim of the study was to evaluate the content of defensin1 in honeys of different botanical origins and to investigate a presumed effect of reactive MGO on defensin1 and a dominant protein of honey MRJP1 in manuka honey. Immunoblotting of honey samples showed that defensin1 was a regular but quantitatively variable component of honeys. One of the reasons of varying contents of defensin1 in different honeys seems to be constitutive but varying defensin1 expression in individual honeybees in bee populations that we documented on samples of nurse and forager bees by RT-PCR. Comparative analyses of honeys revealed a size modification of defensin1, MRJP1 and probably also α-glucosidase in manuka honey. We further showed that (i) the treatment of purified defensin1 in solution containing high amount of MGO caused a time-dependent loss of its antibacterial activity and (ii) increasing MGO concentrations in a non-manuka honey were connected with a gradual increase in the molecular weight of MRJP1. Obtained results demonstrate that MGO abrogates the antibacterial activity of defensin1 and modifies MRJP1 in manuka honey. We assume that MGO could also have negative effects on the structure and function of other proteins/peptides in manuka honey, including glucose oxidase, generating hydrogen peroxide. [Display omitted]
ISSN:0367-326X
DOI:10.1016/j.fitote.2012.02.002